Voltage-dependent removal of sodium inactivation by N-bromoacetamide and pronase.
Voltage-dependent removal of sodium inactivation by N-bromoacetamide and pronase.
复制标题
N-溴乙酰胺和链霉酶对钠失活的电压依赖性去除。
DOI：
10.1016/s0006-3495(85)83952-7
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发表时间：
1985
影响因子：
3.4
通讯作者：
Narahashi,T
中科院分区：
文献类型：
--
作者：
Salgado,VL;Yeh,JZ;Narahashi,T
当通过小龙虾巨型轴突内部灌流时，链霉酶在-100 mV时清除钠灭活的速度是-30 mV时的三倍多。在膜内施加N-溴乙酰胺，在-100 mV时对钠灭活的去除速度是-30 mV时的两倍，膜去极化的相对去除速率与稳态钠灭活成正比。我们得出结论，在封闭的构象中，钠灭活门部分地被N-溴乙酰胺和链霉酶破坏。
When perfused internally through crayfish giant axons, pronase removed sodium inactivation more than three times as fast at -100 mV as compared with -30 mV. N-bromoacetamide, applied internally, removed sodium inactivation twice as fast at -100 mV as at -30 mV, and the relative rate of removal declined with membrane depolarization in proportion to steady-state sodium inactivation. We conclude that in the closed conformation the sodium inactivation gate is partially protected from destruction by N-bromoacetamide and pronase.