CIRCULATING TUMOR-NECROSIS-FACTOR, INTERLEUKIN-1 AND INTERLEUKIN-6 CONCENTRATIONS IN CHRONIC-ALCOHOLIC PATIENTS
CIRCULATING TUMOR-NECROSIS-FACTOR, INTERLEUKIN-1 AND INTERLEUKIN-6 CONCENTRATIONS IN CHRONIC-ALCOHOLIC PATIENTS
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DOI：
10.1016/0270-9139(91)92439-f
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发表时间：
1991-02-01
期刊：
影响因子：
13.5
通讯作者：
ALLEN, JI
中科院分区：
文献类型：
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作者：
KHORUTS, A;STAHNKE, L;ALLEN, JI
尽管细胞因子稳态的改变与酒精性肝病的发病机制有关，但细胞因子与酒精性肝病代谢后果之间的关系尚不清楚。因此，我们试图将肿瘤坏死因子-α、白细胞介素-1 和白细胞介素-6 的循环浓度与慢性酒精患者肝病的临床和生化参数相关联。 我们使用酶联免疫吸附测定法测量血浆肿瘤坏死因子和白细胞介素-1，并使用生物测定法测量三组酗酒男性的血清白细胞介素-6，如下：（a）没有慢性肝病证据的积极饮酒的酗酒男性，（b）患有稳定肝硬化的不饮酒酗酒男性和（c）患有急性酒精性肝炎的患者。 与对照组和无肝病的酗酒患者相比，肝硬化患者和酒精性肝炎患者的平均细胞因子浓度升高。诊断酒精性肝炎后，肿瘤坏死因子-α 和白细胞介素 1-α 浓度在长达 6 个月内保持升高状态，而白细胞介素 6 在临床恢复的同时恢复正常。所有三种细胞因子的浓度均与肝损伤和肝蛋白合成以及血清免疫球蛋白浓度的生化参数相关。我们无法证明细胞因子浓度与外周内毒素血症之间的关系。 酒精患者和对照组的外周血单核细胞与 CD25（白细胞介素 2 受体）单克隆抗体和人淋巴细胞抗原 DR 发生反应的百分比相似。 这些数据表明，肿瘤坏死因子-α 和白介素-1-α 与急性和慢性酒精性肝病的一些代谢后果有关，而白细胞介素-6 与急性肝损伤中出现的异常有关。
Although altered cytokine homeostasis has been implicated in the pathogenesis of alcoholic liver disease, the relationship between cytokines and metabolic consequences of alcoholic liver disease is unknown. We, therefore, sought to correlate circulating concentrations of tumor necrosis factor-alpha, interleukin-1 and interleukin-6 to clinical and biochemical parameters of liver disease in chronic alcoholic patients. We used an enzyme-linked immunosorbent assay to measure plasma tumor necrosis factor and interleukin-1 and a bioassay to measure serum interleukin-6 in three groups of alcoholic men as follows: (a) actively drinking alcoholic men without evidence of chronic liver disease, (b) nondrinking alcoholic men with stable cirrhosis and (c) patients with acute alcoholic hepatitis. Mean cytokine concentrations were elevated in cirrhotic patients and alcoholic hepatitis patients compared with controls and alcoholic patients without liver disease. Tumor necrosis factor-alpha and interleukin-1-alpha concentrations remained elevated for up to 6 mo after diagnosis of alcoholic hepatitis, whereas interleukin-6 normalized in parallel with clinical recovery. Concentrations of all three cytokines were correlated with biochemical parameters of liver injury and hepatic protein synthesis plus serum immunoglobulin concentrations. We could not demonstrate a relationship between cytokine concentrations and peripheral endotoxemia. Percentages of peripheral blood monocytes that reacted with monoclonal antibodies to CD25 (interleukin-2-receptor) and human lymphocyte antigen-DR were similar for alcoholic patients and controls. These data suggest that tumor necrosis factor-alpha and interleukin-1-alpha are related to some of the metabolic consequences of both acute and chronic alcohol-induced liver disease, whereas interleukin-6 is related to abnormalities seen in acute liver injury.