Changes in the expression of gallinacins, antimicrobial peptides, in ovarian follicles during follicular growth and in response to lipopolysaccharide in laying hens (Gallus domesticus)
Changes in the expression of gallinacins, antimicrobial peptides, in ovarian follicles during follicular growth and in response to lipopolysaccharide in laying hens (Gallus domesticus)
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DOI：
10.1530/rep-06-0083
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发表时间：
2007-01-01
期刊：
影响因子：
3.8
通讯作者：
Yoshimura, Yukinori
中科院分区：
文献类型：
--
作者：
Subedi, Kalpana;Isobe, Naoki;Yoshimura, Yukinori
本研究的目的是确定在卵泡中表达的gallinacin基因（GALs）的类型，并确定其表达的变化在卵泡生长过程中，并响应于脂多糖（LPS）。从产蛋鸡（n=5）和注射LPS的母鸡（n=3）收集不同生长阶段的卵泡。半定量RT-PCR检测GALs在卵泡膜层和颗粒层的表达。在白色和黄色卵泡中，在卵泡膜层中鉴定出GAL-1、GAL-2、GAL-7、GAL- 8、GAL-10和GAL-12，在颗粒层中鉴定出GAL-1、GAL- 8、GAL-10和GAL-12。这些基因的表达没有改变卵泡膜和颗粒层在卵泡生长过程中，除了减少GAL-1在卵泡膜。LPS（1 mg/kg体重）可诱导第三大卵泡卵泡膜层GAL 1、GAL-7和GAL-12的表达增加，这种增加在3 h内被诱导，并持续到注射后12 h。颗粒层直到12 h才对LPS有反应。这些结果表明，6种和4种类型的GAL分别在健康卵泡的卵泡膜层和颗粒层中表达，并且除了卵泡膜中的GAL-1外，它们的水平不随卵泡生长而变化。响应于LPS，卵泡膜中GAL-1、GAL-7和GAL-12表达水平升高表明表达这些GAL的卵泡膜细胞具有消除含LPS细菌的功能。
The aim of this study was to identify the types of gallinacin genes (GALs) expressed in ovarian follicles and to determine the changes in their expression during follicular growth and in response to lipopolysaccharide (LPS). Follicles at different stages of growth were collected from laying hens (n=5) and LPS-injected hens (n=3). The expression of GALs in the theca and granulosa layers was examined by semi-quantitative RT-PCR. The expression of GAL-1, -2, -7,-8, -10, and -12 in the theca layer and GAL-1, - 8, -10, and -12 in the granulosa layer was identified in white and yellow follicles. The expression of these genes was not changed in the theca and granulosa layers during follicular growth except for a decrease in that of GAL-1 in theca. The expression of GAL1, -7, and -12 in the theca layer of the third largest follicles was increased in response to LPS at a dose of 1 mg/kg body weight and this increase was induced within 3 h and maintained until 12h postinjection. Granulosa layers did not respond to LPS until 12h injection. These results show that six and four types of GALs are expressed in the theca and granulosa layers of healthy follicles respectively, and their levels do not change with follicular growth except for GAL-1 in theca. Elevated levels of GAL-1, -7, and -12 expression in theca in response to LPS suggest that the theca cells expressing these GALs function to eliminate LPS-containing bacteria.