Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells
Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells
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DOI：
10.7150/ijms.8.56
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发表时间：
2011-01-01
影响因子：
3.6
通讯作者：
Huang, Yu-Zheng
中科院分区：
文献类型：
--
作者：
Huang, Xue-Wen;Luo, Rui-Hua;Huang, Yu-Zheng
为探讨活性氧（ROS）在幽门螺杆菌（Helicobacter pylori，Hp）诱导线粒体DNA（mtDNA）突变中的作用，用Hp 11638和Hp 11638 M的提取物处理AGS细胞。测量ROS水平、细胞色素C减少和细胞内ATP水平。扩增编码区和D-Loop区并测序。结果显示，两种Hp提取物处理后，细胞内活性氧水平、细胞色素C还原和线粒体DNA突变均明显增加，细胞活力下降，D-Loop区检测到616个突变，Cytb基因检测到3个异质点突变。编码区未发现突变。线粒体D-Loop区突变率与ROS水平呈正相关，与ATP水平呈负相关。
To investigate the role of ROS in the helicobacter pylori (Hp) induced mtDNA mutations, AGS cells were treated by extracts of Hp11638 or Hp11638M. The ROS levels, cytochrome C reductions, and intracellular ATP levels were measured. The coding region and the D-Loop region were amplified and sequenced. Results showed the ROS levels, cytochrome C reduction and mtDNA mutations were markedly increased and cell viability decreased after treatment with both Hp extracts, and 616 mutations were detected in D-Loop region and 3 heteroplasmic point mutations in the Cytb gene. No mutations were found in the coding region. The mutation rates of mtDNA D-Loop region were positively correlated with the ROS levels and negatively to the ATP levels.