Expression of the atypical protein kinase C (aPKC) isoforms ι/λ and ζ during mouse embryogenesis
Expression of the atypical protein kinase C (aPKC) isoforms ι/λ and ζ during mouse embryogenesis
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DOI：
10.1016/j.modgep.2006.07.002
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发表时间：
2007-01-01
影响因子：
1.2
通讯作者：
Leitges, Michael
中科院分区：
文献类型：
--
作者：
Kovac, Judit;Oster, Henrik;Leitges, Michael
非典型C型蛋白激酶（aPKC）包括PKC家族的第三个亚类，其功能由对佛波醇酯、二酰基甘油和钙的不敏感性定义。aPKC参与许多生物学过程，包括细胞增殖和存活、细胞极性、迁移和炎症。然而，关于aPKC亚型特异性的数据不足，因为哺乳动物aPKC，PKCi/lambda和PKC zeta，表现出高度的结构同源性和非常相似的生化特性。因此，在这项研究中，我们使用亚型特异性核糖核酸探针和抗体来确定小鼠胚胎发生过程中每个aPKC亚型的特征性表达谱。PKCi/lambda和zeta都显示出高度特异性的时空表达模式，这可能有助于区分这些亚型的生理功能。(c)2006 Elsevier B. V.保留所有权利。
The atypical C-type protein kinases (aPKCs) comprise the third subclass of the PKC family functionally defined by insensitivity to phorbol esters, diacylgylcerol and calcium. aPKCs have been implicated in numerous biological processes including cell proliferation and survival, cell polarity, migration and inflammation. However, only insufficient data exist with regard to aPKC isoform specificity, since both mammalian aPKCs, PKCi/lambda and PKC zeta, exhibit a high structural homology and very similar biochemical properties. In this study, we therefore used isoform-specific riboprobes and antibodies to define the characteristic expression profile of each aPKC isoform during mouse embryogenesis. Both, PKCi/lambda and zeta show highly specific temporal and spatial patterns of expression which may help in distinguishing physiological functions of these isoforms. (c) 2006 Elsevier B.V. All rights reserved.