Resistance of Gram-positive bacteria to nisin is not determined by Lipid II levels
Resistance of Gram-positive bacteria to nisin is not determined by Lipid II levels
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DOI：
10.1016/j.femsle.2004.08.033
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发表时间：
2004-10-01
影响因子：
2.1
通讯作者：
Breukink, E
中科院分区：
文献类型：
--
作者：
Kramer, NE;Smid, EJ;Breukink, E
脂质II在纳摩尔浓度下对于乳链菌肽介导的孔形成是必需的。我们测试了乳酸链球菌素耐药性是否可以导致不同的脂质II水平，通过比较最大的脂质II池在微球菌黄（敏感）和李斯特菌（相对不敏感）和它们的乳酸链球菌素耐药的变种，与一个新开发的方法。在最大脂质II库和乳链菌肽敏感性之间没有观察到相关性，这通过使用乳链菌肽抗性和野生型M. flavus，对乳链菌肽同样敏感。(C)2004年，欧洲微生物学会联合会。Elsevier B.V.出版，保留所有权利。
Lipid II is essential for nisin-mediated pore formation at nano-molar concentrations. We tested whether nisin resistance could result from different Lipid II levels, by comparing the maximal Lipid II pool in Micrococcus flavus (sensitive) and Listeria monocytogenes (relatively insensitive) and their nisin-resistant variants, with a newly developed method. No correlation was observed between the maximal Lipid II pool and nisin sensitivity, as was further corroborated by using spheroplasts of nisin-resistant and wild-type strains of M. flavus, which were equally sensitive to nisin. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.