REGION-SPECIFIC REGULATION OF PREPROENKEPHALIN MESSENGER-RNA IN CULTURED ASTROCYTES
REGION-SPECIFIC REGULATION OF PREPROENKEPHALIN MESSENGER-RNA IN CULTURED ASTROCYTES
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DOI：
10.1016/0169-328x(91)90022-p
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发表时间：
1991-08-01
期刊：
影响因子：
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通讯作者：
KESSLER, JA
中科院分区：
文献类型：
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作者：
BATTER, DK;KESSLER, JA
在新生大鼠脑的几个区域培养的星形胶质细胞中检测了前脑啡肽（PPE） mRNA的调控。这些区域的星形胶质细胞表达不同水平的PPE mRNA，下丘脑的星形胶质细胞表达水平较高，其次是额叶皮层和纹状体。此外，PPE mRNA在下丘脑中的调控与纹状体胶质中的调控不同。用-肾上腺素能激动剂异丙肾上腺素或直接增加细胞内cAMP（福斯可林或8-溴cAMP）的药物治疗纹状体星形胶质细胞，升高PPE mRNA水平。相比之下，这些处理均未改变下丘脑星形胶质细胞中PPE mRNA的水平，尽管cAMP水平增加了60倍。这些观察结果表明，星形胶质细胞对PPE mRNA的表达和调控存在显著的区域异质性，表明前脑啡肽或其衍生肽有助于介导区域特异性脑功能。
Regulation of preproenkephalin (PPE) mRNA was examined in astrocytes cultured from several regions of the neonatal rat brain. Astrocytes from these regions expressed differing levels of PPE mRNA, with higher levels in astrocytes from the hypothalamus followed by frontal cortex and striatum. Further, PPE mRNA was regulated differently in hypothalamic than in striatal glia. Treatment of striatal astrocytes with the beta-adrenergic agonist, isoproterenol, or with agents which directly increased intracellular cAMP (forskolin or 8-bromo-cAMP) elevated levels of PPE mRNA. By contrast, none of these treatments altered levels of PPE mRNA in hypothalamic astrocytes despite increasing cAMP levels 60-fold. These observations indicate that there is striking regional heterogencity in the expression and regulation of PPE mRNA by astrocytes, suggesting that proenkephalin or its derived peptides help to mediate region-specific brain functions.