Presence of luteinizing hormone-releasing hormone (LHRH) mRNA in rat spleen lymphocytes.
Presence of luteinizing hormone-releasing hormone (LHRH) mRNA in rat spleen lymphocytes.
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DOI：
10.1210/endo-128-3-1679
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发表时间：
1991-03
期刊：
影响因子：
4.8
通讯作者：
N. Azad;N. Emanuele;M. M. Halloran-M.;J. Tentler;M. Kelley
中科院分区：
文献类型：
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作者：
N. Azad;N. Emanuele;M. M. Halloran-M.;J. Tentler;M. Kelley
根据我们在大鼠脾脏淋巴细胞中发现的免疫反应性和生物活性的促黄体生成素释放激素（LHRH）样分子，我们试图通过测定淋巴细胞是否含有LHRH mRNA来确定这些细胞是否能够合成LHRH。为此，从下丘脑组织、垂体前叶和淋巴细胞中提取总RNA，然后将其反向转录为cDNA，并通过聚合酶链反应（PCR）利用合成的寡核苷酸包裹LHRH基因的一部分进行扩增。凝胶电泳在下丘脑（阳性对照）和淋巴细胞中发现了375个碱基对大小的离散条带，但在垂体前叶（阴性对照）中没有发现。此外，Southern blotting后，32p标记的LHRH cDNA在下丘脑片段和淋巴细胞中扩增，杂交到375个碱基对，但在垂体前叶组织中没有扩增。这些数据有力地表明，LHRH除了是一种重要的神经肽外，还是一种免疫细胞合成的免疫调节剂。
Pursuant to our report of an immunoreactive and bioactive luteinizing hormone-releasing hormone (LHRH)-like molecule in rat spleen lymphocytes, we sought to determine whether these cells were capable of synthesizing LHRH by determining whether lymphocytes contain LHRH mRNA. To do this, total RNA was extracted from hypothalamic tissue, anterior pituitaries and from lymphocytes, and then this was reverse transcribed to cDNA and amplified via the polymerase chain reaction (PCR) utilizing synthetic oligonucleotides bracketing a portion of the LHRH gene. Following gel electrophoresis a discrete band of the expected size of 375 base pairs was found in the hypothalamus (positive control), and in lymphocytes, but not in the anterior pituitary (negative control). Furthermore, after Southern blotting, a 32P-labelled LHRH cDNA, hybridized to the 375 base pair, was amplified in hypothalamus fragments and in lymphocytes, but not in anterior pituitary tissue. These data strongly suggest that LHRH, in addition to being an important neuropeptide, is an immune cell synthesized immunomodulator.