IDENTIFICATION OF THE HPV-16 E6 PROTEIN FROM TRANSFORMED MOUSE CELLS AND HUMAN CERVICAL-CARCINOMA CELL-LINES
IDENTIFICATION OF THE HPV-16 E6 PROTEIN FROM TRANSFORMED MOUSE CELLS AND HUMAN CERVICAL-CARCINOMA CELL-LINES
复制标题
DOI：
10.1002/j.1460-2075.1987.tb04849.x
复制
发表时间：
1987-04-01
期刊：
影响因子：
11.4
通讯作者：
LOWY, DR
中科院分区：
文献类型：
--
作者：
ANDROPHY, EJ;HUBBERT, NL;LOWY, DR
携带人乳头瘤病毒（HPV）的人宫颈癌细胞系已被报道选择性地保留并表达可编码病毒E6和E7蛋白的HPV序列。牛乳头瘤病毒（BPV）E6在体外可诱导小鼠细胞发生形态学转化，这进一步表明HPV E6对肿瘤的潜在重要性。为了鉴定HPV E6蛋白，在细菌表达载体中产生由HPV-16 E6编码的多肽并用于产生抗血清。抗血清特异性免疫沉淀预测的18 kd蛋白在两个人癌细胞系已知表达HPV-16 RNA和小鼠细胞形态学转化的HPV-16 DNA。18 kd的E6蛋白不同于以前鉴定的HPV-16 E7蛋白。发现HPV-16 E6抗体是类型特异性的，因为它们不识别含有HPV-18序列的细胞中的E6蛋白，并且对BPV E6蛋白反应弱，如果有的话。结果表明，含有HPV-16 DNA的人肿瘤可以表达E6蛋白产物。它们与E6可能有助于表达该蛋白的人类宫颈癌中的转化表型的假设一致。
Human cervical carcinoma cell lines that harbor human papillomavirus (HPV) have been reported to retain selectively and express HPV sequences which could encode viral E6 and E7 proteins. The potential importance of HPV E6 to tumors is suggested further by the observation that bovine papillomavirus (BPV) E6 can induce morphologic transformation of mouse cells in vitro. To identify HPV E6 protein, a polypeptide encoded by HPV-16 E6 was produced in a bacterial expression vector and used to raise antisera. The antisera specifically immunoprecipitated the predicted 18-kd protein in two human carcinoma cell lines known to express HPV-16 RNNA and in mouse cells morphologically transformed by HPV-16 DNA. The 18-kd E6 protein was distinct from a previously identified HPV-16 E7 protein. The HPV-16 E6 antibodies were found to be type specific in that they did not recognize E6 protein in cells containing HPV-18 sequences and reacted weakly, if at all, to BPV E6 protein. The results demonstrate that human tumors containing HPV-16 DNA can express an E6 protein product. They are consistent with the hypothesis that E6 may contribute to the transformed phenotype in humnan cervical cancers that express this protein.