The absorption of tritium‐labelled pyridoxine hydrochloride in the rat
The absorption of tritium‐labelled pyridoxine hydrochloride in the rat
复制标题
氚标记盐酸吡哆醇在大鼠体内的吸收
DOI：
--
复制
发表时间：
1962
期刊：
影响因子：
--
通讯作者：
M. Brain
中科院分区：
文献类型：
--
作者：
C. Booth;M. Brain
直到最近，才有可能通过测量相对大剂量口服后吡哆醇及其代谢物的尿排泄来间接研究吡哆醇的吸收。Scudi，Unna & Antopol（1940）和Scudi，Koones & Keresztesy（1940）在人、狗和大鼠中进行的初步研究使用比色法估计吡哆醇及其代谢物。虽然这种方法无法估计4-吡哆酸，现在已知4-吡哆酸是吡哆醇在人体内的主要尿代谢物（Reddy，Reynolds & Price，1958），但这些工作人员确实表明吡哆醇在摄入后很快就从尿液中排出，因此他们得出结论，吸收必须非常迅速。最近，氚标记的盐酸吡哆醇已经上市，现在可以准确地测量少量的这种物质。本文报道了氚化吡哆醇在大鼠体内吸收的研究结果。对不同剂量的吸收速率和部位进行了观察;还研究了大鼠小肠吸收越来越多吡哆醇的能力。
Until recently it was only possible to study th eabsorption of pyridoxine indirectly by measuring the urinary excretion of pyridoxine and its metabolites following relatively large oral doses. The initial studies of Scudi, Unna & Antopol (1940) and Scudi, Koones & Keresztesy (1940) in man, the dog and the rat, were made using a colorimetic method for estimating pyridoxine and its metabolites. Although this method fails to estimate 4-pyridoxic acid, now known to be the principal urinary metabolite ofpyridoxine in man (Reddy, Reynolds & Price, 1958), these workers did show that pyridoxine was excreted in the urine very soon after it was ingested, and they therefore concluded that absorption must be exceedingly rapid. Recently pyridoxine hydrochloride labelled with tritium has become available, and it is now possible to measure small amounts of this substance with accuracy. In this paper we report the results of studies of absorption of tritiated pyridoxine in the rat. Observations were made on the rates and sites of absorption of varying doses; the capacity of the small intestine of the rat to absorb increasing amounts of pyridoxine was also studied.