Insulin-like growth factors and their binding proteins in the term and preterm human fetus and neonate with normal and extremes of intrauterine growth.
Insulin-like growth factors and their binding proteins in the term and preterm human fetus and neonate with normal and extremes of intrauterine growth.
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DOI：
10.1210/jcem.80.5.7538146
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发表时间：
1995-05
期刊：
影响因子：
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通讯作者：
L. Giudice;F. de Zegher;S. Gargosky;B. Dsupin;L. de las Fuentes;R. Crystal;R. Hintz;R. Rosenfeld
中科院分区：
文献类型：
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作者：
L. Giudice;F. de Zegher;S. Gargosky;B. Dsupin;L. de las Fuentes;R. Crystal;R. Hintz;R. Rosenfeld
胰岛素样生长因子（IGFs）、胰岛素样生长因子结合蛋白（IGFBPs）和胰岛素被认为在调节胎儿和新生儿生长方面很重要。我们先前报道了胎儿脐带血清（FCS）中IGFBPs的分布依赖于胎儿的生长/代谢状态。本研究的目的是检测正常生长的足月胎儿、宫内生长迟缓（IUGR）胎儿和大于胎龄（LGA）胎儿的FCS中的IGF系统，以及正常体重早产（25-37周）胎儿和新生儿期（出生当天（0天）至7天（7天））足月胎儿的FCS中的IGF系统。终末FCS的蛋白质配体印迹法（WLB）显示IGFBP分子量为43和38千道尔顿（kDa; IGFBP-3）、34 kDa（IGFBP-2）、28 kDa（IGFBP-1和糖基化IGFBP-4）和24 kDa（IGFBP-4）。在IUGR FCS中，WLB检测到IGFBP-3减少了50%，这表明不是由于IUGR血清中的IGFBP-3蛋白酶引起的。在LGA FCS中，通过配体印迹的光密度分析，IGFBP-3水平升高2倍。在正常足月FCS中，存在以下水平（+/- SE）：IGF-I，76 +/- 16; IGF-II，401 +/- 38; IGFBP-3，700 +/- 112; IGFBP-1，77 +/- 10 ng/mL;胰岛素，3.8 +/- 1.6 microU/mL。在IUGR FCS中，IGF-I、IGF-II和IGFBP-3显著降低，IGFBP-1是正常体重胎儿FCS的7倍。在LGA FCS中，IGF-I、胰岛素和IGFBP-3显著升高，而IGFBP-1显著降低。在新生儿期，与早产儿（25-31周）相比，足月（38-40周）FCS中第0天的IGF-I水平高4倍。在妊娠25-40周之间，第0天FCS IGF-II水平没有显著变化。在出生后的前7天，早产儿的IGF-I水平没有变化，而在足月新生儿中，IGF-I水平在第1天急剧下降，在出生后的前3天保持较低水平，并在第一周结束时恢复到出生水平。相比之下，早产儿或足月新生儿出生后第一周内IGF-II和IGFBP-3水平没有显着变化。（400字处截断摘要）
Insulin-like growth factors (IGFs), IGF-binding proteins (IGFBPs), and insulin are believed to be important in the regulation of fetal and neonatal growth. We previously reported that the profiles of IGFBPs in fetal cord serum (FCS) were dependent on the growth/metabolic status of the fetus. The goals of the current study were to examine the IGF system in FCS from term fetuses with normal growth, those with intrauterine growth retardation (IUGR), and those who were large for gestational age (LGA) and in FCS from normal weight preterm (25-37 weeks) and term fetuses in the neonatal period from the day of birth (day 0) until 7 days of age (day 7). Western ligand blotting (WLB) of term FCS revealed IGFBPs with mol wt of 43 and 38 kilodaltons (kDa; IGFBP-3), 34 kDa (IGFBP-2), 28 kDa (IGFBP-1 and glycosylated IGFBP-4), and 24 kDa (IGFBP-4). In IUGR FCS, there was a 50% decrease in IGFBP-3 detected by WLB, which was shown not to be due to an IGFBP-3 protease in IUGR sera. In LGA FCS, IGFBP-3 levels were elevated 2-fold by densitometric analysis of ligand blots. In normal term FCS, the following levels (+/- SE) were present: IGF-I, 76 +/- 16; IGF-II, 401 +/- 38; IGFBP-3, 700 +/- 112; IGFBP-1, 77 +/- 10 ng/mL; and insulin, 3.8 +/- 1.6 microU/mL. In IUGR FCS, IGF-I, IGF-II, and IGFBP-3 were significantly reduced, and IGFBP-1 was 7-fold higher than in FCS from normal weight fetuses. In LGA FCS, IGF-I, insulin, and IGFBP-3 were significantly increased, whereas IGFBP-1 was significantly decreased. During the neonatal period, IGF-I levels on day 0 were 4-fold higher in FCS from term (38-40 weeks) compared to preterm (25-31 weeks) newborns. FCS IGF-II levels did not change significantly on day 0 between 25-40 weeks gestation. In the first 7 days of postnatal life, IGF-I levels were unchanged in preterm newborns, whereas in term neonates, IGF-I levels decreased precipitously on day 1, remained low during the first 3 days of life, and returned to birth levels by the end of the first week. In contrast, IGF-II and IGFBP-3 levels did not significantly change during the first week of life in preterm or term newborns.(ABSTRACT TRUNCATED AT 400 WORDS)