The Brazilian Amazonian rainforest harbors a high diversity of yeasts associated with rotting wood, including many candidates for new yeast species
The Brazilian Amazonian rainforest harbors a high diversity of yeasts associated with rotting wood, including many candidates for new yeast species
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DOI：
10.1002/yea.3837
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发表时间：
2023-01-22
期刊：
影响因子：
2.6
通讯作者：
Rosa，Carlos A.
中科院分区：
文献类型：
--
作者：
Barros，Katharina O.;Alvarenga，Flavia B. M.;Rosa，Carlos A.
本研究调查了巴西亚马逊雨林中与腐烂木材相关的酵母菌种类的多样性。从亚马逊州Itacoatiara市三个亚马逊地区（Universidade Federal do Amazonas [UFAM]、piquiade Federal do Amazonas [UFAM]、piquiade <e:1>和Carú）的腐烂木材样本中分离出569株酵母菌。样品分别在酵母氮碱(YNB) - d -木糖、YNB -木聚糖、甘蔗渣和玉米芯半纤维素水解物（未稀释和稀释1:2和1:5）中培养。在这项工作中发现的最普遍的属是杉山属，其次是哈萨克斯坦属。最常见的酵母菌种是eschwanomyces polymorphus、Scheffersomyces amazonensis和wickerhamyessp。,分别。α多样性分析表明，UFAM的旱地森林多样性最多，Carú的漫滩森林多样性最少。此外，在比较中，unfam和Carú之间的多样性差异最大。获得了30个候选酵母菌种，占已鉴定菌种的36%，共101株分离物。其中，木糖发酵酵母属（spathaspora）、木糖发酵菌属（Scheffersomyces）和木糖发酵菌属（sugiyamaella）被认为是天然木糖发酵酵母属，在生物技术和生态学研究中得到广泛应用。这项工作的结果表明，从亚马逊雨林收集的腐烂木材是各种酵母的巨大来源，包括新物种的候选者。
This study investigated the diversity of yeast species associated with rotting wood in Brazilian Amazonian rainforests. A total of 569 yeast strains were isolated from rotting wood samples collected in three Amazonian areas (Universidade Federal do Amazonas‐Universidade Federal do Amazonas [UFAM], Piquiá, and Carú) in the municipality of Itacoatiara, Amazon state. The samples were cultured in yeast nitrogen base (YNB)‐d‐xylose, YNB‐xylan, and sugarcane bagasse and corncob hemicellulosic hydrolysates (undiluted and diluted 1:2 and 1:5).Sugiyamaellawas the most prevalent genus identified in this work, followed byKazachstania. The most frequently isolated yeast species wereSchwanniomyces polymorphus,Scheffersomyces amazonensis, andWickerhamomycessp., respectively. The alpha diversity analyses showed that the dryland forest of UFAM was the most diverse area, while the floodplain forest of Carú was the least. Additionally, the difference in diversity between UFAM and Carú was the highest among the comparisons. Thirty candidates for new yeast species were obtained, representing 36% of the species identified and totaling 101 isolates. Among them were species belonging to the cladesSpathaspora,Scheffersomyces, andSugiyamaella, which are recognized as genera with natural xylose‐fermenting yeasts that are often studied for biotechnological and ecological purposes. The results of this work showed that rotting wood collected from the Amazonian rainforest is a tremendous source of diverse yeasts, including candidates for new species.