The induction of cytolytic T lymphocytes with purified plasma membranes.
The induction of cytolytic T lymphocytes with purified plasma membranes.
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用纯化的质膜诱导溶细胞 T 淋巴细胞。
DOI：
10.4049/jimmunol.120.4.1114
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发表时间：
1978
影响因子：
4.4
通讯作者：
S. Burakoff
中科院分区：
文献类型：
--
作者：
F. Lemonnier;T. Mescher;L. Sherman;S. Burakoff
亚细胞组分被证明能有效刺激原发性和继发性异基因CTL反应。用5′核苷酸酶活性来评价质膜的纯化，发现刺激活性与细胞的质膜部分共纯化。这些纯化质膜的电子显微照片显示，大部分物质以相对不均匀大小的囊泡形式存在。纯化质膜培养产生的T淋巴细胞具有免疫特异性。
Subcellular fractions were demonstrated to be effective in stimulating primary and secondary allogeneic CTL responses. 5' nucleotidase activity was used to assess purification of plasma membranes and stimulating activity was found to co-purify with the plasma membrane fraction of the cell. Electron micrographs of these purified plasma membranes showed the majority of the material to be in the form of vesicles of relatively heterogeneous size. The cytolytic T lymphocytes generated by incubation with purified plasma membranes demonstrated immunologic specificity.