A paper-based in vitro model for on-chip investigation of the human respiratory system.
A paper-based in vitro model for on-chip investigation of the human respiratory system.
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DOI：
10.1039/c6lc00866f
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发表时间：
2016-11
期刊：
影响因子：
6.1
通讯作者：
R. Rahimi;S. Htwe;M. Ochoa;Amy Donaldson;M. Zieger;R. Sood;A. Tamayol;A. Khademhosseini;A. Ghaemm
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文献类型：
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作者：
R. Rahimi;S. Htwe;M. Ochoa;Amy Donaldson;M. Zieger;R. Sood;A. Tamayol;A. Khademhosseini;A. Ghaemm
在空气-液体界面（ALI）处培养细胞对于创建肺组织的功能性体外模型是必不可少的。我们提出使用直接图案化的激光处理的疏水纸作为有效的半透膜，理想的ALI细胞培养。纸的表面特性通过选择性CO2激光辅助处理进行改性，以创建具有调节流体扩散和细胞附着的亲水区域的独特多孔基材。为了选择合适的模型，四个有前途的疏水膜进行了比较，彼此在气体渗透性和长期强度在水性环境（湿强度）。在所研究的基材中，羊皮纸的透氧速率最快（比传统的transwell细胞培养膜高3倍），干、湿拉伸强度变化最小（124 MPa和58 MPa，在PBS中浸没7天后保持不变）。和廉价的装置，用于以高通量方式在芯片上产生单层肺上皮细胞，用于疾病建模和体外药物测试。
Culturing cells at the air-liquid interface (ALI) is essential for creating functional in vitro models of lung tissues. We present the use of direct-patterned laser-treated hydrophobic paper as an effective semi-permeable membrane, ideal for ALI cell culture. The surface properties of the paper are modified through a selective CO2 laser-assisted treatment to create a unique porous substrate with hydrophilic regions that regulate fluid diffusion and cell attachment. To select the appropriate model, four promising hydrophobic films were compared with each other in terms of gas permeability and long-term strength in an aqueous environment (wet-strength). Among the investigated substrates, parchment paper showed the fastest rate of oxygen permeability (3 times more than conventional transwell cell culture membranes), with the least variation in its dry and wet tensile strengths (124 MPa and 58 MPa, remaining unchanged after 7 days of submersion in PBS).The final paper-based platform provides an ideal, robust, and inexpensive device for generating monolayers of lung epithelial cells on-chip in a high-throughput fashion for disease modelling and in vitro drug testing.