LncRNA-LOC101928316 contributes to gastric cancer progression through regulating PI3K-Akt-mTOR signaling pathway
LncRNA-LOC101928316 contributes to gastric cancer progression through regulating PI3K-Akt-mTOR signaling pathway
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LncRNA-LOC101928316通过调节PI3K-Akt-mTOR信号通路促进胃癌进展
DOI：
10.1002/cam4.2165
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发表时间：
2019-08-01
期刊：
影响因子：
4
通讯作者：
Zhang, Yan
中科院分区：
文献类型：
--
作者：
Li, Chengyun;Liang, Geyu;Zhang, Yan
长链非编码RNA （Long noncoding RNA, lncRNA）在多种生物过程的调控中发挥着重要作用，在诊断方面的价值得到了广泛的认识。在本研究中，我们发现LOC101928316在胃癌（GC）组织标本、胃癌细胞系中显著下调，并与胃癌患者肿瘤、淋巴结和转移（TNM）分期及分化程度相关（P & xff1c; 0.05）。LOC101928316过表达可显著抑制SGC-7901细胞的迁移、侵袭和增殖（P & xff1c;0.05）。对LOC101928316分子机制的研究表明，LOC101928316可以调节PI3K-Akt-mTOR信号通路，改变体内外GC的发育进程。体内实验还发现，过表达loc101928316可抑制肿瘤负荷实验裸鼠GC细胞的致瘤性（P & xff1c; 0.05）。LOC101928316可能具有抑癌作用，在胃癌发生过程中也起重要作用。总的来说，我们的数据提供了LOC101928316在GC肿瘤发生中的关键作用。此外，本研究阐明了LOC101928316的潜在调控网络，这可能有助于我们更好地了解GC的发病机制，并探索lncRNA作为一种新的生物标志物来诊断和治疗这种恶性肿瘤。
Long noncoding RNA (lncRNA) has played the important function in regulation of various biological processes and in diagnostic value has been widely appreciated. In the present study, we have found that LOC101928316 was significantly downregulated in gastric cancer (GC) tissues specimen, GC cell lines, and associated with the GC patients tumor, node, and metastasis (TNM) stage and degree of differentiation (P & xff1c; 0.05). LOC101928316 overexpression can significantly inhibit SGC-7901 cell migration, invasion, and proliferation (P & xff1c;0.05). LOC101928316 molecular mechanism investigates suggested that LOC101928316 can regulate PI3K-Akt-mTOR signaling pathway and change the GC development progression in vivo and in vitro. In vivo experiment also revealed that LOC101928316-Overexpression can inhibit the tumorigenicity of GC cells in tumor-burdened experimental nude mice (P & xff1c; 0.05). LOC101928316 may function as anti-oncogene and also plays an important role in GC tumorigenesis. Collectively, our data provided the key role of LOC101928316 in the tumorigenesis of GC. In addition, the present study elucidates LOC101928316 potential regulatory network, which may help us to lead a better knowing of the pathogenesis of GC and probe the lncRNA as a novel biomarker to diagnosis and therapy for this malignant tumor.