Organoid culture systems for prostate epithelial and cancer tissue.
Organoid culture systems for prostate epithelial and cancer tissue.
复制标题
DOI：
10.1038/nprot.2016.006
复制
发表时间：
2016-02
期刊：
影响因子：
14.8
通讯作者：
Clevers H
中科院分区：
文献类型：
--
作者：
Drost J;Karthaus WR;Gao D;Driehuis E;Sawyers CL;Chen Y;Clevers H
该方案描述了最近开发的从健康小鼠和人前列腺（散装或FAC-sorted单腔和基底细胞）、转移性前列腺癌病变和循环肿瘤细胞生成3D前列腺类器官培养物的策略。来源于健康材料的类器官含有分化的管腔和基底细胞类型，而来源于前列腺癌组织的类器官模拟肿瘤的组织学。逐步建立这些文化和完全定义的无血清条件培养基，需要维持类器官的生长概述。使用该方案建立的类器官可用于研究前列腺生物学的许多不同方面，包括稳态，肿瘤发生和药物发现。
This protocol describes a recently developed strategy to generate 3D prostate organoid cultures from healthy mouse and human prostate (either bulk or FAC-sorted single luminal and basal cells), metastatic prostate cancer lesions and circulating tumour cells. Organoids derived from healthy material contain the differentiated luminal and basal cell types, whereas organoids derived from prostate cancer tissue mimic the histology of the tumour. The stepwise establishment of these cultures and the fully defined serum-free conditioned medium that is required to sustain organoid growth are outlined. Organoids established using this protocol can be used to study many different aspects of prostate biology, including homeostasis, tumorigenesis and drug discovery.