Synergistic effect of ultrasound and antibiotics against Chlamydia trachomatis-infected human epithelial cells in vitro
Synergistic effect of ultrasound and antibiotics against Chlamydia trachomatis-infected human epithelial cells in vitro
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DOI：
10.1016/j.ultsonch.2010.07.015
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发表时间：
2011-01-01
影响因子：
8.4
通讯作者：
Maruyama, Kazuo
中科院分区：
文献类型：
--
作者：
Ikeda-Dantsuji, Yurika;Feril, Loreto B., Jr.;Maruyama, Kazuo
为了研究超声波和抗生素联合治疗对衣原体感染是否有效，设计了一种新的超声波照射系统来治疗衣原体感染的细胞。首先，确定了抗生素对沙眼衣原体的最低抑菌浓度。感染的培养物用抗生素处理，然后以0.15或0.44W/cm(2)的强度超声，加或不加泡泡脂质体。感染后48h或72h取衣原体包涵体染色，在荧光显微镜下观察。在荧光显微镜下观察超声辐照泡沫脂质体对葡聚糖-荧光素结合物的内化作用。结果表明，应用纳米气泡超声对细胞存活率和衣原体感染性无明显影响。然而，与单独使用抗生素相比，强力霉素(1/2MIC)或CZX(1.0mU g/ml)与纳米气泡超声联合使用显著减少了包裹体的数量。气泡剂量依赖的协同效应也被观察到。经0.44W/cm2的超声辐照后，观察到10%的HeLa细胞内化了葡聚糖分子。这项研究表明，使用纳米气泡增强超声波将抗生素分子输送到细胞内，以辐射细胞内的细菌，如衣原体，而不会对细胞本身造成太大损害的可能性。(C)2010爱思唯尔B.V.保留所有权利。
To investigate whether or not the combined ultrasound and antibiotic treatment is effective against chlamydial infection, a new ultrasound exposure system was designed to treat chlamydia-infected cells. First, the minimum inhibitory concentrations of antibiotics against Chlamydia trachomatis were determined. Infected cultures were treated with antibiotics then sonicated at intensity of 0.15 or 0.44 W/cm(2) with or without Bubble liposomes. After 48 or 72 h after infection, chlamydial inclusions were stained and examined by fluorescence microscopy. The internalization of dextran-fluorescein conjugates by ultrasound irradiation with Bubble liposomes was observed by fluorescence microscopy. The results showed that application of nanobubble-enhanced ultrasound caused no significant effect on cell viability and chlamydial infectivity. However, Doxycycline (1/2 MIC) or CZX (1.0 mu g/ml) in combination with nanobubble-enhanced ultrasound dramatically reduced the number of inclusions compared with that administered with antibiotics only. Bubble dose-dependent synergy was also observed. After ultrasound irradiation at intensity of 0.44 W/cm(2) on the presence of Bubble liposomes, 10% of HeLa cells were observed to have internalized the dextran molecules. This study suggests the possibility of using nanobubble-enhanced ultrasound to deliver antibiotic molecules into cells to eradiate intracellular bacteria, such as chlamydiae, without causing much damage to the cells itself. (C) 2010 Elsevier B.V. All rights reserved.