Source of trait deterioration in entomopathogenic nematodes Heterorhabditis bacteriophora and Steinernema carpocapsae during in vivo culture
Source of trait deterioration in entomopathogenic nematodes Heterorhabditis bacteriophora and Steinernema carpocapsae during in vivo culture
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DOI：
10.1163/156854106778493394
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发表时间：
2006-01-01
期刊：
影响因子：
1.2
通讯作者：
Adams, Byron J.
中科院分区：
文献类型：
--
作者：
Bilgrami, Anwar L.;Gaugler, Randy;Adams, Byron J.
研究了致病性线虫-细菌复合体异杆菌和斯氏线虫对生物防治重要性状的稳定性。每个物种的 5 个实验品系在大蜡螟幼虫中传代培养 20 代，以评估性状稳定性。传代培养会损害 H. bacteriophora 和 S. carpocapsae 的性能。所有H. bacteriophora 实验品系的毒力、耐热性和繁殖力均下降，并且五分之四的实验品系的宿主发现能力下降。所有 S. carpocapsae 实验品系的耐热性和眨眼能力均恶化，五分之四的实验品系的繁殖能力下降，而两个实验品系的毒力下降。通过将来自对照群体的线虫或细菌与来自最恶化实验品系的线虫或细菌交换并评估性状恢复来测试性状恶化是否是由于线虫、细菌或两个共生伙伴的变化所致。恶化的来源根据性状的不同而不同，但只有细菌伙伴在每个性状和物种的性状降低中发挥了作用，而线虫只是 S. carpocapsae nictation 的主要来源。这些结果强调了每个共生伙伴在有益性状的稳定性和表达中发挥的重要作用。
The stability of traits important for biological control was studied in the entornopathogenic nematode-bacteria complexes Heterorhabditis bacteriophora and Steinernema carpocapsae. Five experimental lines of each species were subcultured for 20 serial passages in Galleria mellonella larvae to assess trait stability. Subculturing impaired performance of both H. bacteriophora and S. carpocapsae. Virulence, heat tolerance and fecundity deteriorated in all H. bacteriophora experimental lines, and four out of five experimental lines deteriorated in host-finding ability. All S. carpocapsae experimental lines deteriorated in heat tolerance and nictation, and four out of five experimental lines declined for reproductive capacity, whereas virulence declined in two experimental lines. Determination of whether trait deterioration was due to changes in nematode, bacteria, or both symbiotic partners was tested by exchanging nematodes or bacteria from control populations with nematodes or bacteria from the most deteriorated experimental lines and assessing trait recovery. The source of deterioration varied according to trait, but only the bacterial partner played a role in trait reductions for every trait and species, whereas the nematode was the main source only for S. carpocapsae nictation. These results emphasise the important role each symbiotic partner plays in the stability and expression of beneficial traits.