Regulation of lipid droplet size in mammary epithelial cells by remodeling of membrane lipid composition-a potential mechanism.
Regulation of lipid droplet size in mammary epithelial cells by remodeling of membrane lipid composition-a potential mechanism.
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通过重塑膜脂质成分 - 潜在机制来调节乳腺上皮细胞中脂质液滴的大小。
DOI：
10.1371/journal.pone.0121645
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Argov-Argaman N
中科院分区：
文献类型：
--
作者：
Cohen BC;Shamay A;Argov-Argaman N
乳脂肪球的大小由其前体（细胞内脂滴）的大小决定，并与其成分密切相关。我们检查了乳腺上皮细胞的磷脂组成与细胞内和分泌的乳脂肪球大小之间的关系。将乳腺上皮细胞的原代培养物在不含游离脂肪酸（对照）或含有 0.1 mM 游离癸酸、棕榈酸或油酸的培养基中培养 24 小时。测定细胞和培养基中细胞脂质的量和组成以及脂滴的大小。测定了与线粒体生物发生和极性脂质组成相关的基因的线粒体数量和表达水平。用油酸和棕榈酸培养的细胞含有相似数量的甘油三酯，分别比对照高 3.1 倍和 3.8 倍（P < 0.0001）。当用油酸培养时，22%的细胞含有大脂滴（>3μm），并且与对照和棕榈酸处理相比，磷脂酰乙醇胺浓度分别高出23%和63%（P < 0.0001）。在棕榈酸存在下，只有 4% 的细胞含有大脂滴，膜磷脂酰胆碱浓度分别比对照和油酸处理高 22% 和 16% (P < 0.0001)。在油酸处理中，大约 40% 的脂滴大于 5 μm，而在棕榈酸处理中，只有 16% 的脂滴在此尺寸范围内。与棕榈酸和对照处理相比，油酸处理中的甘油三酯分泌分别高2倍和12倍。结果表明，牛乳腺上皮细胞的膜组成在控制细胞内和分泌的脂滴大小方面发挥着作用，并且该过程与细胞甘油三酯含量无关。
Milk fat globule size is determined by the size of its precursors—intracellular lipid droplets—and is tightly associated with its composition. We examined the relationship between phospholipid composition of mammary epithelial cells and the size of both intracellular and secreted milk fat globules. Primary culture of mammary epithelial cells was cultured in medium without free fatty acids (control) or with 0.1 mM free capric, palmitic or oleic acid for 24 h. The amount and composition of the cellular lipids and the size of the lipid droplets were determined in the cells and medium. Mitochondrial quantity and expression levels of genes associated with mitochondrial biogenesis and polar lipid composition were determined. Cells cultured with oleic and palmitic acids contained similar quantities of triglycerides, 3.1- and 3.8-fold higher than in controls, respectively (P < 0.0001). When cultured with oleic acid, 22% of the cells contained large lipid droplets (>3 μm) and phosphatidylethanolamine concentration was higher by 23 and 63% compared with that in the control and palmitic acid treatments, respectively (P < 0.0001). In the presence of palmitic acid, only 4% of the cells contained large lipid droplets and the membrane phosphatidylcholine concentration was 22% and 16% higher than that in the control and oleic acid treatments, respectively (P < 0.0001). In the oleic acid treatment, approximately 40% of the lipid droplets were larger than 5 μm whereas in that of the palmitic acid treatment, only 16% of the droplets were in this size range. Triglyceride secretion in the oleic acid treatment was 2- and 12-fold higher compared with that in the palmitic acid and control treatments, respectively. Results imply that membrane composition of bovine mammary epithelial cells plays a role in controlling intracellular and secreted lipid droplets size, and that this process is not associated with cellular triglyceride content.
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