Mutual Interactions Between GnRH and Kisspeptin in GnRH- and Kiss-1-Expressing Immortalized Hypothalamic Cell Models
Mutual Interactions Between GnRH and Kisspeptin in GnRH- and Kiss-1-Expressing Immortalized Hypothalamic Cell Models
复制标题
表达 GnRH 和 Kiss-1 的永生化下丘脑细胞模型中 GnRH 和 Kisspeptin 之间的相互作用
DOI：
10.1007/s43032-021-00695-z
复制
发表时间：
2021
期刊：
影响因子：
2.9
通讯作者：
Kyo S.
中科院分区：
文献类型：
--
作者：
Kanasaki H;Tumurbaatar T;Tumurgan Z;Oride A;Okada H;Kyo S.
Kisspeptin and gonadotropin-releasing hormone (GnRH) are central regulators of the hypothalamic-pituitary-gonadal axis and control female reproductive functions. Recently established mHypoA-50 and mHypoA-55 cells are immortalized hypothalamic neuronal cell models that originated from the anteroventral periventricular nucleus (AVPV) and arcuate nucleus (ARC) regions of the mouse hypothalamus, respectively. mHypoA-50 or mHypoA-55 cells were stimulated with kisspeptin-10 (KP10) and GnRH, after which the expression of kisspeptin and GnRH was determined. Primary cultures of fetal rat brain cells were also examined. mHypoA-50 and mHypoA-55 cells expressed mRNA for Kiss-1 (which encodes kisspeptin) and GnRH as well as receptors for kisspeptin and GnRH. We found that Kiss-1 mRNA expression was significantly increased in mHypoA-50 AVPV cells by KP10 and GnRH stimulation. Kisspeptin protein expression was also increased by KP10 and GnRH stimulation in these cells. In contrast, GnRH expression was unchanged in mHypoA-50 AVPV cells by KP10 and GnRH stimulation. In mHypoA-55 ARC cells, kisspeptin expression was also significantly increased at the mRNA and protein levels by KP10 and GnRH stimulation; however, GnRH expression was also upregulated by KP10 and GnRH stimulation in these cells. KP10 and estradiol (E2) both increased Kiss-1 gene expression in mHypoA-50 AVPV cells, but combined stimulation with KP10 and E2 did not potentiate their individual effects on Kiss-1 gene expression. On the other hand, E2 did not increase Kiss-1 gene expression in mHypoA-55 ARC cells, and the KP10-induced increase of Kiss-1 gene expression was inhibited in the presence of E2 in these cells. KP10 and GnRH significantly increased c-Fos protein expression in the mHypoA-50 AVPV and mHypoA-55 ARC cell lines. In primary cultures of fetal rat neuronal cells, KP10 significantly increased Kiss-1 gene expression, whereas GnRH significantly increased GnRH gene expression. We found that kisspeptin and GnRH affected Kiss-1- and GnRH-expressing hypothalamic cells and modulated Kiss-1 and/or GnRH gene expression with a concomitant increase in c-Fos protein expression. A mutual- or self-regulatory system might be present in Kiss-1 and/or GnRH neurons in the hypothalamus.
登录
查看更多内容
下丘脑促性腺激素释放激素神经元中功能性 g 蛋白偶联受体 54-Kisspeptin 自动调节系统的表达。
DOI：
10.1210/me.2007-0207
发表时间：
2007
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Quaynor,Samuel;Hu,Lian;Leung,PoKi;Feng,Hao;Mores,Nadia;Krsmanovic,LazarZ;Catt,KevinJ
通讯作者：
Catt,KevinJ
DOI：
10.1097/00006254-200405000-00020
发表时间：
2004-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
S. Seminara;S. Messager;E. Chatzidaki;R. Thresher;J. Acierno;Jenna K Shagoury;Y. Bo-Abbas;W. Kuohung-W.-Ku
通讯作者：
S. Seminara;S. Messager;E. Chatzidaki;R. Thresher;J. Acierno;Jenna K Shagoury;Y. Bo-Abbas;W. Kuohung-W.-Ku
DOI：
10.1073/pnas.0409822102
发表时间：
2005-02-08
影响因子：
11.1
作者：
Shahab, M;Mastronardi, C;Plant, TM
通讯作者：
Plant, TM
雄性和雌性大鼠视前神经元中含有降钙素基因相关肽的雌激素受体的免疫细胞化学鉴定。
影响因子：
4.1
作者：
A. Herbison;D. Theodosis
通讯作者：
D. Theodosis
影响因子：
3.3
作者：
Ciofi, P.;Leroy, D.;Tramu, G.
通讯作者：
Tramu, G.