ACUTE DELTA-9-TETRAHYDROCANNABINOL EXPOSURE - EFFECTS ON HYPOTHALAMIC PITUITARY TESTICULAR ACTIVITY IN MICE
ACUTE DELTA-9-TETRAHYDROCANNABINOL EXPOSURE - EFFECTS ON HYPOTHALAMIC PITUITARY TESTICULAR ACTIVITY IN MICE
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DOI：
10.1016/0091-3057(87)90161-4
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发表时间：
1987-03-01
影响因子：
3.6
通讯作者：
DEPAOLO, L
中科院分区：
文献类型：
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作者：
DALTERIO, S;STEGER, R;DEPAOLO, L
在体内或体外暴露于Δ 9-四氢大麻酚（THC）后，在雄性小鼠中检查正中隆起、垂体和睾丸的内分泌功能。在基础条件下和存在去甲肾上腺素时促黄体生成素释放激素（LHRH）的分泌（东北; 60 μ M），在用THC处理后1小时获得的正中隆起碎片中显著增强（50 mg/kg），而加入THC（250 ng/ml）的孵育介质中增加可乐定，以及NE刺激的LHRH释放，但不影响基础LHRH释放。在体外暴露于THC也增强LHRH刺激的垂体LH释放，但不影响基础分泌率。体内THC暴露倾向于增强垂体对LHRH的反应性，尽管这种作用没有统计学意义。在睾丸灌流中，加入浓度为250 ng/ml的THC在30 min内完全阻断hCG刺激的T分泌。较低剂量的THC（25 ng/ml）的抑制作用需要60 min才能抑制T产生，THC后这种作用持续60-80 min。这些发现表明，THC暴露增强神经内分泌靶位点的反应性，但减弱促性腺激素刺激的睾丸类固醇生成。
The endocrine functions of the median eminence, pituitary and testes were examined in male mice after exposure to .DELTA.9-tetrahydrocannabinol (THC) either in vivo or in vitro. The secretion of luteinizing hormone-releasing hormone (LHRH) under basal conditions, and in the presence of norepinephrine (NE; 60 .mu.M), was significantly enhanced in median eminence fragments obtained 1 hr post-treatment with THC (50 mg/kg), while addition of THC (250 ng/ml) to the incubation media enhanced clonidine, as well as NE-stimulated LHRH release, but did not affect basal LHRH release. In vitro exposure to THC also enhanced LHRH-stimulated LH release by pituitaries, but did not affect basal secretion rates. In vivo THC exposure tended to enhance pituitary responsiveness to LHRH, although this effect was not statistically significant. In testicular perifusions, addition of THC at a concentration of 250 ng/ml completely blocked hCG-stimulated T secretion within 30 min. The suppressive effects of a lower dose of THC, 25 ng/ml, required 60 min to inhibit T production, an effect which persisted for 60-80 min post-THC. These findings indicate that THC exposure enhances responsivity at neuroendocrine target sites, but attenuates gonadotropin-stimulated testicular steroidogenesis.