GENETIC EXPRESSION FOR TYPE-I PROCOLLAGEN IN THE EARLY STAGES OF FLEXOR TENDON HEALING
GENETIC EXPRESSION FOR TYPE-I PROCOLLAGEN IN THE EARLY STAGES OF FLEXOR TENDON HEALING
复制标题
DOI：
10.1016/0363-5023(92)90370-5
复制
发表时间：
1992-05-01
影响因子：
1.9
通讯作者：
HARWOOD, F
中科院分区：
文献类型：
--
作者：
GELBERMAN, RH;AMIEL, D;HARWOOD, F
为了确定接触肌腱愈合在细胞水平上发生的精确机制，通过原位杂交技术测定了愈合的滑膜内屈肌腱成纤维细胞产生的前α（I）胶原信使RNA（mRNA）的产生。修复后3、7、10和17天，将修复部位和早期控制性被动活动治疗的修复肌腱的近端和远端肌腱残端固定并在福尔马林中缓冲。用[P-32]d-CTP标记对应于α（I）前胶原mRNA的互补DNA（cDNA）探针。杂交、放射自显影和切片染色后，通过显微镜检查评估前胶原mRNA的水平。上升的前胶原mRNA水平，表明逐步增加的合成胶原活性水平，检测到愈合肌腱通过10天。在第17天，前胶原mRNA中度减少。前胶原mRNA的基因表达定位于肌腱表面上覆盖修复部位的腱外膜细胞和肌腱残端之间差距的细胞。在腱内成纤维细胞中未观察到可检测的表达。在愈合肌腱的表面层中高水平表达I型前胶原mRNA的发现表明，肌腱肌腱外膜固有的细胞在肌腱缝合后的这些重要的早期间隔期间向修复部位贡献了最大量的天然肌腱胶原。
To determine the precise mechanism by which contact tendon healing occurs at the cellular level, the production of pro alpha(I) collagen messenger RNA (mRNA) produced by fibroblasts of healing intrasynovial flexor tendons was determined by an in situ hybridization technique. The repair site and the proximal and distal tendon stumps of repaired tendons treated with early controlled passive mobilization were fixed and buffered in formalin, 3, 7, 10, and 17 days after repair. A complimentary DNA (cDNA) probe corresponding to alpha(I) procollagen mRNA was labeled with [P-32]d-CTP. After hybridization, autoradiography, and staining of the sections, the level of procollagen mRNA was assessed by microscopic examination. Rising levels of procollagen mRNA, indicating progressively increasing levels of synthetic collagen activity, were detected in the healing tendons through 10 days. A moderate decrease in procollagen mRNA was seen at 17 days. Genetic expression for procollagen mRNA was localized specifically to the epitenon cells on the tendon surface overlying the repair site and to cells in the gap between the tendon stumps. No detectable expression was noted in endotenon fibroblasts. The finding of high levels of expression for procollagen type I mRNA in the surface layer of healing tendons demonstrates that cells intrinsic to tendon epitenon contribute the greatest quantity of native tendon collagen to the repair site during these important early intervals after tendon suture.