Biocatalysis
Biocatalysis
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DOI：
10.1038/s43586-021-00044-z
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发表时间：
2021-06-24
期刊：
影响因子：
--
通讯作者：
Flitsch, Sabine L.
中科院分区：
文献类型：
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作者：
Bell, Elizabeth L.;Finnigan, William;Flitsch, Sabine L.
生物催化已经成为现代有机合成的一个重要方面，无论是在学术界还是在化学和制药工业中。它的成功在很大程度上是由于可访问的化学反应范围的快速扩展，通过酶发现的先进工具以及用于生物催化剂优化的高通量实验室进化技术而成为可能。现在可以快速生产各种具有高效率和高选择性的定制酶，并以克到千克的规模生产，专用数据库和搜索工具旨在使这些生物催化剂可供更广泛的科学界使用。本书讨论了目前该领域的最先进的方法，包括路线设计，酶的发现，蛋白质工程和生物催化在工业中的实施。我们强调了最近的进展，如从头设计和定向进化，并讨论了参数，使一个良好的可重复的生物催化过程的工业。一般的概念将说明在学术界和工业界，包括多步酶级联的发展最近的应用程序的例子。
Biocatalysis has become an important aspect of modern organic synthesis, both in academia and across the chemical and pharmaceutical industries. Its success has been largely due to a rapid expansion of the range of chemical reactions accessible, made possible by advanced tools for enzyme discovery coupled with high-throughput laboratory evolution techniques for biocatalyst optimization. A wide range of tailor-made enzymes with high efficiencies and selectivities can now be produced quickly and on a gram to kilogram scale, with dedicated databases and search tools aimed at making these biocatalysts accessible to a broader scientific community. This Primer discusses the current state-of-the-art methodology in the field, including route design, enzyme discovery, protein engineering and the implementation of biocatalysis in industry. We highlight recent advances, such as de novo design and directed evolution, and discuss parameters that make a good reproducible biocatalytic process for industry. The general concepts will be illustrated by recent examples of applications in academia and industry, including the development of multistep enzyme cascades.