Differential proteomic analysis of four near-isogenic Brassica napus varieties bred for their erucic acid and glucosinolate contents
Differential proteomic analysis of four near-isogenic Brassica napus varieties bred for their erucic acid and glucosinolate contents
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DOI：
10.1021/pr060450b
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Larre, Colette
中科院分区：
文献类型：
--
作者：
Devouge, Vanessa;Rogniaux, Helene;Larre, Colette
4个近等基因型B。具有反映实际育种过程的降低量的芥酸和芥子油苷的油菜品种被用于表征在该过程中受影响的蛋白质。改进2-DE条件后，通过质谱分析鉴定差异积累的蛋白质。十字花科植物中十字花科植物素的积累仅受到轻微的影响，而显著的数量差异主要表现在参与防御系统和碳水化合物代谢的蛋白质。
Four near-isogenic B. napus varieties, with decreasing amounts of erucic acid and glucosinolates reflecting the actual breeding process, were used to characterize the proteins affected during this process. Following improvement of 2-DE conditions, proteins differentially accumulated were identified by mass spectrometry analysis. Accumulation of cruciferins was found to be only slightly affected, whereas significant quantitative differences were mainly found for proteins involved in defense system and carbohydrate metabolism.