Systematic analysis reveals novel insight into the molecular determinants of function, diversity and evolution of sweet taste receptors T1R2/T1R3 in primates.
Systematic analysis reveals novel insight into the molecular determinants of function, diversity and evolution of sweet taste receptors T1R2/T1R3 in primates.
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DOI：
10.3389/fmolb.2023.1037966
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发表时间：
2023
影响因子：
5
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中科院分区：
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甜味是灵长类动物对食物偏好和适应的主要感觉，对灵长类动物的生存和健康至关重要。目前已经清楚的是，甜味感知是由G蛋白偶联受体（GPCR）-甜味受体T1 R2/T1 R3介导的，并且许多行为或生理实验已经描述了灵长类动物的多种甜味敏感性。然而，灵长类动物中T1 R2 s/T1 R3 s的结构-功能关系，特别是其物种依赖性甜味的分子基础，迄今为止尚未得到很好的理解。在这项研究中，我们进行了全面的序列，结构和功能分析的甜味受体在灵长类动物，以阐明介导的分子决定因素，其物种依赖性的甜味识别。我们的研究结果揭示了独特的分类分布和显着的特征（相互作用，协同进化和上位性）的特定的关键功能相关的残基，这可以部分解释先前报道的灵长类动物的味觉感知的行为结果。此外，原猴目狐猴形目物种，据报道，没有敏感性，以阿贝甜，可以提出的基础上，本分析的阿贝甜品尝。总的来说，我们的研究提供了新的见解，并促进了对灵长类动物甜味受体的多样性，功能和进化的更好理解。
Sweet taste is a primary sensation for the preference and adaption of primates to diet, which is crucial for their survival and fitness. It is clear now that the sweet perception is mediated by a G protein-coupled receptor (GPCR)-sweet taste receptor T1R2/T1R3, and many behavioral or physiological experiments have described the diverse sweet taste sensitivities in primates. However, the structure-function relationship of T1R2s/T1R3s in primates, especially the molecular basis for their species-dependent sweet taste, has not been well understood until now. In this study, we performed a comprehensive sequence, structural and functional analysis of sweet taste receptors in primates to elucidate the molecular determinants mediating their species-dependent sweet taste recognition. Our results reveal distinct taxonomic distribution and significant characteristics (interaction, coevolution and epistasis) of specific key function-related residues, which could partly account for the previously reported behavioral results of taste perception in primates. Moreover, the prosimians Lemuriformes species, which were reported to have no sensitivity to aspartame, could be proposed to be aspartame tasters based on the present analysis. Collectively, our study provides new insights and promotes a better understanding for the diversity, function and evolution of sweet taste receptors in primates.