Artificial cells: Unique insights into exocytosis using liposomes and lipid nanotubes
Artificial cells: Unique insights into exocytosis using liposomes and lipid nanotubes
复制标题
DOI：
10.1073/pnas.232702599
复制
发表时间：
2003-01-21
影响因子：
11.1
通讯作者：
Ewing, A
中科院分区：
文献类型：
--
作者：
Cans, AS;Wittenberg, N;Ewing, A
胞吐作用是神经元通讯的基本过程。这一过程包括一个含有神经递质的小泡与细胞质膜融合，释放出微量的递质分子。胞吐作用被认为经历了一个中间步骤，包括形成小的脂质纳米管或融合孔，然后孔扩张到胞吐作用的最终阶段。已经通过各种方法研究了胞吐作用的过程;然而，当使用活细胞时，难以区分膜蛋白相对于脂质膜驱动过程的机制的分子效应，并且难以操纵系统参数（例如，膜组成、pH、离子浓度、温度等）。我们描述了使用脂质体-脂质纳米管网络来创建经历胞吐后期阶段的人工细胞模型。该模型表明，在没有蛋白质干预的情况下，膜力学可以驱动融合孔的扩张到胞吐的最后阶段，并且可以影响通过融合孔释放递质的速率。
Exocytosis is the fundamental process underlying neuronal communication. This process involves fusion of a small neurotransmitter-containing vesicle with the plasma membrane of a cell to release minute amounts of transmitter molecules. Exocytosis is thought to go through an intermediate step involving formation of a small lipid nanotube or fusion pore, followed by expansion of the pore to the final stage of exocytosis. The process of exocytosis has been studied by various methods; however, when living cells are used it is difficult to discriminate between the molecular effects of membrane proteins relative to the mechanics of lipid-membrane-driven processes and to manipulate system parameters (e.g., membrane composition, pH, ion concentration, temperature, etc.). We describe the use of liposome-lipid nanotube networks to create an artificial cell model that undergoes the later stages of exocytosis. This model shows that membrane mechanics, without protein intervention, can drive expansion of the fusion pore to the final stage of exocytosis and can affect the rate of transmitter release through the fusion pore.