The Bulge Area Is the Origin of Nestin-Expressing Pluripotent Stem Cells of the Hair Follicle
The Bulge Area Is the Origin of Nestin-Expressing Pluripotent Stem Cells of the Hair Follicle
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DOI：
10.1002/jcb.23122
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发表时间：
2011-08-01
影响因子：
4
通讯作者：
Hoffman, Robert M.
中科院分区：
文献类型：
--
作者：
Uchugonova, Aisada;Duong, Jennifer;Hoffman, Robert M.
在隆起区(BA)和真皮乳头(DP)中都发现了表达巢蛋白的多能干细胞。表达巢蛋白的BA和DP干细胞此前已被证明能够形成神经元和其他非滤泡细胞类型。来自DP的表达Nestin的干细胞被称为皮肤前体细胞或SKP细胞。研究表明，表达巢蛋白的DP细胞和BA细胞都能修复受损的脊髓和周围神经，BA是干细胞的更大和更稳定的来源。BA在整个毛发周期中都含有表达巢蛋白的干细胞，而表达巢蛋白的毛乳头干细胞仅见于生长期早期和中期。我们以前的研究表明，BA和DP中表达Nestin的干细胞具有相似的形态特征。如双光子成像所示，这两个区域的细胞都有一个小的直径约7毫米的长突起。在本研究中，通过对表达巢蛋白驱动的绿色荧光蛋白(ND-GFP)的转基因小鼠的胡须毛囊进行双光子成像，我们证明BA是毛囊中表达巢蛋白的干细胞的来源。在伤口愈合过程中，表达巢蛋白的干细胞从BA向DP以及周围皮肤组织(包括表皮)迁移，提示BA可能是皮肤本身干细胞的来源。J.细胞。生物化学。112：2046-2050,2011。(C)2011年Wiley-Liss，Inc.
Nestin-expressing pluripotent stem cells have been found both in the bulge area (BA) as well as the dermal papilla (DP). Nestin-expressing stem cells of both the BA and DP have been previously shown to be able to form neurons and other non-follicle cell types. The nestin-expressing stem cells from the DP have been termed skin precursor or SKP cells. Both nestin-expressing DP and BA cells have been previously shown to effect repair of the injured spinal cord and peripheral nerve, with the BA being the greater and more constant source of the stem cells. The BA contains nestin-expressing stem cells throughout the hair cycle, whereas nestin-expressing dermal papillae stem cells were found in early and mid-anagen only. Our previous studies have shown that the nestin-expressing stem cells in the BA and DP have similar morphological features. The cells from both regions have a small body diameter of approximately 7 mm with long extrusions, as shown by 2-photon imaging. In the present study, using 2-photon imaging of whisker follicles from transgenic mice expressing nestin-driven green fluorescent protein (ND-GFP), we demonstrate that the BA is the source of the nestin-expressing stem cells of the hair follicle. The nestin-expressing stem cells migrate from the BA to the DP as well as into the surrounding skin tissues including the epidermis, and during wound healing, suggesting that the BA may be the source of the stem cells of the skin itself. J. Cell. Biochem. 112: 2046-2050, 2011. (C) 2011 Wiley-Liss, Inc.