Cerebral ischemia after bilateral carotid artery occlusion and intraluminal suture occlusion in mice: Evaluation of the patency of the posterior communicating artery
Cerebral ischemia after bilateral carotid artery occlusion and intraluminal suture occlusion in mice: Evaluation of the patency of the posterior communicating artery
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DOI：
10.1097/00004647-199805000-00012
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发表时间：
1998-05-01
影响因子：
6.3
通讯作者：
Yanagihara, T
中科院分区：
文献类型：
--
作者：
Kitagawa, K;Matsumoto, M;Yanagihara, T
使用小鼠的脑缺血模型已经引起越来越多的关注，特别是因为转基因动物的可用性。然而，小鼠Willis环处颅内血管的变异性可影响双侧颈总动脉（CCA）闭塞和腔内缝合闭塞模型中的缺血程度。我们已经开发了一种方法来预测颈动脉和椎基底动脉循环之间的吻合程度在三个小鼠品系（C57 BL/6，CBA和DBA/2）通过测量皮质微灌注与激光多普勒血流仪在1分钟闭塞的CCA。当动物在双侧CCA闭塞期间显示出小于12%的残余皮质微灌注时，小鼠显示出功能性吻合缺失，在闭塞期间额叶皮质中出现ATP耗竭，并且在闭塞15分钟和再循环7天后海马和尾壳中出现缺血性神经元死亡。此外，在管腔内缝合闭塞模型中，这些小鼠在大脑后动脉供应的区域内的海马中表现出局部脑血流量减少和相关的缺血性神经元死亡。目前的研究表明，需要在每只动物的颅内血管系统的评估，通过测量皮质微灌注暂时闭塞的CCA，无论脑缺血是由双侧CCA闭塞或管腔内缝合闭塞在转基因小鼠。
Cerebral ischemia models using mice have drawn increasing attention, particularly because of the availability of transgenic animals. However, the variability of intracranial vasculature at the circle of Willis in mice can influence the degree of ischemia in both the bilateral common carotid artery (CCA) occlusion and intraluminal suture occlusion models. We have developed a method to predict the extent of the anastomosis between carotid and vertebrobasilar circulation in three mouse strains (C57BL/6, CBA, and DBA/2) by measuring cortical microperfusion with laser Doppler flowmetry during a 1-minute occlusion of both CCA. When animals showed residual cortical microperfusion of less than 12% during bilateral CCA occlusion, the mice showed absence of functional anastomosis, developed ATP depletion in the frontal cortex during occlusion, and had ischemic neuronal death in the hippocampus and caudoputamen after occlusion for 15 minutes and recirculation for 7 days. Furthermore, those mice exhibited decreased local cerebral blood flow and associated ischemic neuronal death in the hippocampus, within the territory supplied by the posterior cerebral artery, with the intraluminal suture occlusion model. The current study demonstrates the need for assessment of intracranial vasculature in each animal by measuring cortical microperfusion during temporary occlusion of both CCA, no matter whether cerebral ischemia is produced by bilateral CCA occlusion or intraluminal suture occlusion in transgenic mice.