Preparation of plasmonic vesicles from amphiphilic gold nanocrystals grafted with polymer brushes.
Preparation of plasmonic vesicles from amphiphilic gold nanocrystals grafted with polymer brushes.
复制标题
用聚合物刷接枝的两亲性金纳米晶体制备等离子体囊泡。
DOI：
10.1038/nprot.2016.137
复制
发表时间：
2016-11
期刊：
影响因子：
14.8
通讯作者：
中科院分区：
文献类型：
--
作者：
金纳米囊泡在聚合物涂层内含有多个纳米晶体。相邻纳米粒子在其囊泡壳中的强等离子体激元耦合使得超灵敏的生物传感和生物成像成为可能。在我们的实验室中，多功能等离子体囊泡由涂覆有混合的亲水和疏水聚合物刷或两亲性二嵌段共聚物刷的两亲性金纳米晶体（如金纳米颗粒和金纳米棒）组装而成。为了满足生物医学应用的不同要求，可以使用pH=响应性、光活性或生物可降解的不同聚合物来形成疏水刷，同时通过聚乙二醇（PEG）保持亲水性。该方案涵盖了制备，表面功能化和自组装的两亲性金纳米晶体共价接枝聚合物刷。该方案可在2d内完成。描述了两亲性金纳米晶体的制备，所述两亲性金纳米晶体使用“接枝到”方法涂覆有两亲性二嵌段聚合物刷或使用串联“接枝到”和“接枝自”方法涂覆有混合的亲水性和疏水性聚合物刷。我们还提供了详细的程序的pH响应的等离子体金纳米囊泡的制备和表征从两亲性金纳米晶体使用的膜再水化方法，可以在3天内完成。
Gold nanovesicles contain multiple nanocrystals within a polymeric coating. The strong plasmonic coupling between adjacent nanoparticles in their vesicular shell makes ultrasensitive biosensing and bioimaging possible. In our laboratory, multifunctional plasmonic vesicles are assembled from amphiphilic gold nanocrystals (such as gold nanoparticles and gold nanorods) coated with mixed hydrophilic and hydrophobic polymer brushes or amphiphilic diblock co-polymer brushes. To fulfill the different requirements of biomedical applications, different polymers that are either pH=responsive, photoactive or biodegradable can be used to form the hydrophobic brush, while the hydrophilicity is maintained by polyethylene glycol (PEG). This protocol covers the preparation, surface functionalization and self-assembly of amphiphilic gold nanocrystals grafted covalently with polymer brushes. The protocol can be completed within 2 d. The preparation of amphiphilic gold nanocrystals, coated with amphiphilic diblock polymer brushes using a ‘grafting to’ method or mixed hydrophilic and hydrophobic polymer brushes using tandem ‘grafting to’ and ‘grafting from’ methods, is described. We also provide detailed procedures for the preparation and characterization of pH-responsive plasmonic gold nanovesicles from amphiphilic gold nanocrystals using a film-rehydration method that can be completed within ~3 d.