A rapid "off-on" copper-induced AIE active sensor for fluorimetric detection of cysteine
A rapid "off-on" copper-induced AIE active sensor for fluorimetric detection of cysteine
复制标题
用于半胱氨酸荧光检测的快速“关断”铜诱导 AIE 主动传感器
DOI：
10.1016/j.snb.2019.127214
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Liu Weipeng
中科院分区：
文献类型：
--
作者：
Cai Yue;Fang Jingkun;Zhu Hongshuai;Qin Weiwei;Cao Yiran;Yu Huijuan;Shao Guang;Liu Yingju;Liu Weipeng
半胱氨酸（Cys）是一种含硫氨基酸，在生命过程中起着重要作用。Cys含量异常可引发一系列疾病，如发育迟缓、毛发变色、神经系统疾病和心血管疾病等。研究发现，4-（10-{4-[双（4-己氧基-苯基）-氨基]-苯基}-蒽-9-基乙炔基）-苯甲酸（FSD-103）具有聚集诱导发射（AIE）性质，并在1 min内对Cu ~（2+）具有高选择性的荧光猝灭作用。然后，由于Cys与Cu ~（2+）之间的强相互作用，影响了FSD-103与Cu ~（2+）的结合，从而恢复了AIE效应。基于FSD-103-Cu 2+体系，在8 min内实现了一种新型的Cys“关-开”荧光传感器，其荧光强度与Cys在低浓度（1-10 μM）和高浓度（10-17 μM）范围内呈线性关系，检测限为0.2 μM。有趣的是，通过浸泡滤纸，Cys的荧光恢复效应的可视化可以在μM水平上轻松实现。该方法具有良好的选择性，为有机分子在半胱氨酸检测中的应用提供了一种可行的方法。
As a sulfur-containing amino acid, cysteine (Cys) plays a crucial role in life process. Abnormal Cys content can trigger a series of diseases, such as developmental delay, hair discoloration, neurological diseases and cardiovascular diseases. In this work, 4-(10-{4-[Bis-(4-hexyloxy-phenyl)-amino]-phenyl}-anthracen-9-ylethynyl)-benzoic acid (FSD-103) was found to exhibit the aggregation-induced emission (AIE) property and a further fluorescence quench with a high selectivity to Cu2+in 1 min. Then, due to the strong interaction between Cys and Cu2+, the combination of FSD-103 and Cu2+was influenced, which restored the AIE effect. Therefore, based on the FSD-103-Cu2+system, a new “off-on” fluorescence sensor for Cys detection was realized in 8 min, where a linear relationship between the fluorescence intensity and Cys was found in both low concentration range (1–10 μM) and high concentration range (10–17 μM), and the detection limit was 0.2 μM. Interestingly, by soaking a filter paper, the visualization of the fluorescence recovery effect of Cys can be easily realized at the μM level. This method shows acceptable selectivity, which provides a practicable method for the application of organic molecules in Cys detection.