Effect of platelet releasate on bone cell migration and recruitment in vitro
Effect of platelet releasate on bone cell migration and recruitment in vitro
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DOI：
10.1097/00001665-200305000-00006
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发表时间：
2003-05-01
影响因子：
0.9
通讯作者：
Davies, JE
中科院分区：
文献类型：
--
作者：
Oprea, WE;Karp, JM;Davies, JE
血小板和血小板制品作为加速骨内伤口愈合的一种手段在临床上越来越受欢迎。这可能是由活化血小板在损伤部位释放的生长因子通过刺激骨细胞的迁移和增殖在骨再生中发挥作用。在这项研究中，建立了一种新的体外实验来研究从活化血小板浓缩物中收集的血小板释放（PR）对大鼠骨髓源性细胞的影响。将原代培养的大鼠骨髓细胞覆盖在纤维蛋白基质上，并对三维基质内迁移的细胞数量和迁移前沿进行量化。在不同时间点向纤维蛋白凝胶顶部添加PR，导致迁移前沿增加25%，迁移细胞数量增加3.5倍。在增殖研究中，血小板释放也显示对骨细胞有丝分裂作用。迁移和增殖数据的比较表明，PR刺激骨髓细胞迁移的初始募集。该实验进一步确定了大鼠骨髓细胞能够对纤维蛋白基质施加收缩力，并且基质收缩与细胞的迁移活性直接相关。该结果提供了一种潜在的机制来解释为什么具有生物活性的血小板衍生因子能促进骨内伤口愈合。
The use of platelets and platelet products has become increasingly popular clinically as a means of accelerating endosseous wound healing. It is likely that growth factors released by activated platelets at the site of injury play a role in bone regeneration by stimulating the migration and proliferation of bone cells. In this study, a novel in vitro assay was developed to study the effects of platelet releasate (PR) collected from activated platelet concentrate on rat bone marrow-derived cells. Cultures of primary rat bone marrow cells were overlaid with a fibrin matrix, and the number of cells migrating within the three-dimensional matrix and the leading front of migration were quantified. The addition of PR to the top of the fibrin gels at different time points caused a 25% increase in the leading front of migration and a 3.5-fold increase in the number of migrating cells. Platelet releasate was also shown to have a mitogenic effect on bone cells in proliferation studies. Comparison between migration and proliferation data indicated that PR stimulates the initial recruitment of bone marrow cells to migration. This assay further allowed the determination that rat bone marrow cells are capable of exerting contractile forces on fibrin matrices and that matrix contraction is directly related to the migratory activity of cells. The results provide a potential mechanism to explain why biologically active platelet-derived factors enhance endosseous wound healing.