Reporters for sensitive and quantitative measurement of auxin response
Reporters for sensitive and quantitative measurement of auxin response
复制标题
DOI：
10.1038/nmeth.3279
复制
发表时间：
2015-03-01
期刊：
影响因子：
48
通讯作者：
Weijers, Dolf
中科院分区：
文献类型：
--
作者：
Liao, Che-Yang;Smet, Wouter;Weijers, Dolf
激素信号输入和输出的可视化是理解多细胞发育如何调节的关键。植物信号分子生长素触发许多生长和发育反应，但目前的工具缺乏灵敏度或精确度来可视化这些反应。我们开发了一套荧光报告，允许敏感和半定量读出生长素的反应在细胞分辨率在拟南芥。这些通用工具适用于任何可转化的植物物种。
The visualization of hormonal signaling input and output is key to understanding how multicellular development is regulated. The plant signaling molecule auxin triggers many growth and developmental responses, but current tools lack the sensitivity or precision to visualize these. We developed a set of fluorescent reporters that allow sensitive and semiquantitative readout of auxin responses at cellular resolution in Arabidopsis thaliana. These generic tools are suitable for any transformable plant species.