PERSISTENT, TOLERANT OR SUBACUTE INFECTION IN BORNA DISEASE VIRUS-INFECTED RATS
PERSISTENT, TOLERANT OR SUBACUTE INFECTION IN BORNA DISEASE VIRUS-INFECTED RATS
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DOI：
10.1099/0022-1317-64-7-1521
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发表时间：
1983-01-01
影响因子：
3.8
通讯作者：
LUDWIG, H
中科院分区：
文献类型：
--
作者：
HIRANO, N;KAO, M;LUDWIG, H
适应兔的博尔纳病（BD）病毒V株通过1日龄Wistar大鼠的脑内感染进行传代。感染后4周，感染滴度达到108感染单位/克大脑。没有明显的临床症状。持续感染可以用 BD 病毒的适应毒株或野外毒株诱导。通过中和试验鉴定菌株。适应兔的BD病毒对大鼠的毒力随着大鼠传代而增强。第 5 代在 .gtoreq 感染的动物中引起临床症状。 1周龄。患病老鼠中，20-50%死亡。在所有年龄段感染的大鼠的神经元中均可通过免疫组织学检测到病毒特异性抗原。动物在 1 或 2 个月时接种。随着年龄的增长，但新生大鼠却没有出现大脑炎症的迹象。被感染的老鼠产生了特异性抗体。在老年组（1或2月龄感染）中，特别是在幸存的动物中，偶尔会发现高滴度的中和抗体。引发的脾细胞的转移导致亚急性疾病。显然，新生大鼠可以获得持续的、耐受的感染，并且疾病的表达是由免疫因素介导的。
The rabbit-adapted Borna disease (BD) virus strain V was passaged by intracerebral infection of 1 day old Wistar rats. Infectivity titers reached 108 infectious units/g of brain 4 wk after infection. No clinical signs were evident. The persistent infection could be induced with adapted or field strains of BD virus. Strains were identified by neutralization tests. The virulence of the rabbit-adapted BD virus for the rat increased with rat passages. The 5th passage induced clinical symptoms in animals infected at .gtoreq. 1 wk of age. Of the diseased rats, 20-50% died. Virus-specific antigen was detectable immunohistologically in neurons of rats infected at all ages. Animals inoculated at 1 or 2 mo. of age, but not the neonatal rats, showed signs of inflammation in the brain. Infected rats produced specific antibodies. In the older groups (infected at ages of 1 or 2 mo.), and especially in surviving animals, occasionally, neutralizing antibodies with high titer were found. Transfer of primed spleen cells resulted in subacute disease. Evidently, neonatal rats can acquire a persistent, tolerant infection, and expression of disease is mediated by immunological factors.