Blocking SCF/c-Kit signal did not inhibit the proliferation of cultured liver progenitor cells
Blocking SCF/c-Kit signal did not inhibit the proliferation of cultured liver progenitor cells
复制标题
DOI：
10.1080/15216540701541065
复制
发表时间：
2007-01-01
期刊：
影响因子：
4.6
通讯作者：
Hu, Yiping
中科院分区：
文献类型：
--
作者：
Jin, Caixia;Li, Wenlin;Hu, Yiping
卵圆细胞是公认的肝干细胞，在肝癌发生和化学诱导的严重肝损伤过程中增殖。传统上与造血细胞相关的抗原，如c-Kit，已被报道由卵圆细胞表达。以往的研究表明，干细胞因子（SCF）和c-Kit是至关重要的卵圆细胞的发育。然而，SCF/c-Kit信号在卵圆细胞增殖中的作用仍不清楚。最近，我们报道了卵圆细胞来源的肝上皮祖细胞（LEPCs）的建立。在这项工作中，我们发现LEPCs共表达c-Kit及其配体SCF。通过将LEPC暴露于c-Kit抑制剂（STI 571和AG 1296）、SCF、抗SCF中和抗体或通过使用小干扰RNA敲低c-Kit表达来研究SCF/c-Kit信号在LEPC增殖中的参与。我们的数据表明，阻断SCF/c-Kit信号并不抑制LEPCs的增殖，这表明SCF/c-Kit不是卵圆细胞增殖所必需的，至少对于培养的卵圆细胞对应的LEPCs来说是如此。
Oval cells are the putative liver stem cells that proliferate during hepatocarcinogenesis and chemically-induced severe liver injury. Antigens traditionally associated with haematopoietic cells, such as c-Kit, have been reported to be expressed by oval cells. Previous studies suggested that stem cell factor (SCF) and c-Kit were critical to oval cell development. However, the role of SCF/c-Kit signals in oval cell proliferation still remains unclear. Recently, we reported the establishment of oval cell-derived liver epithelial progenitor cells (LEPCs). In this work, we showed LEPCs co-expressed c-Kit and its ligand SCF. The involvement of SCF/c-Kit signals in LEPCs proliferation was investigated either by exposing LEPCs to c-Kit inhibitors (STI571 and AG1296), SCF, anti-SCF neutralized antibody or by using small interfering RNA to knock-down c-Kit expression. Our data demonstrate that blocking SCF/c-Kit signal did not inhibit the proliferation of LEPCs, which suggest SCF/c-Kit is not necessary for the proliferation of oval cells, at least for the cultured oval cell counterpart LEPCs.