SIMULTANEOUS IMMUNOELECTRON MICROSCOPIC VISUALIZATION OF PROTEIN B23 AND C23 DISTRIBUTION IN THE HELA-CELL NUCLEOLUS
SIMULTANEOUS IMMUNOELECTRON MICROSCOPIC VISUALIZATION OF PROTEIN B23 AND C23 DISTRIBUTION IN THE HELA-CELL NUCLEOLUS
复制标题
DOI：
10.1177/37.9.2768807
复制
发表时间：
1989-09-01
影响因子：
3.2
通讯作者：
BUSCH, H
中科院分区：
文献类型：
--
作者：
BIGGIOGERA, M;FAKAN, S;BUSCH, H
核仁内分布的磷蛋白B23和C23的可视化同时通过后包埋免疫电镜在HeLa细胞核仁，使用特异性抗体。数据显示，蛋白质B23和C23共定位于相同的核仁区室，即，致密的纤维状组分和颗粒状组分。这两种抗体均不与这些细胞中的纤维中心显著相关，尽管纤维中心在银染色后呈阳性。这些发现表明，其他不明成分必须负责观察到的纤维中心的间期核仁的银染色。结果进行了讨论，根据先前报道的数据，通过预包埋免疫标记技术和银染色，这都表明本地化的蛋白质C23内的纤维中心。
The intranucleolar distribution of phosphoproteins B23 and C23 was visualized simultaneously by post-embedding immunoelectron microscopy in HeLa cell nucleoli, using specific antibodies. The data show that proteins B23 and C23 co-localize to the same nucleolar compartments, i.e., the dense fibrillar component and the granular component. Neither of the two antibodies is significantly associated with the fibrillar centers in these cells, although the fibrillar centers appear positive after silver staining. These findings suggest that other unidentified components must be responsible for the silver staining observed in the fibrillar centers of interphase nucleoli. The results are discussed in the light of previously reported data obtained by preembedding immunolabeling techiques and by silver staining, which both suggested a localization of protein C23 inside the fibrillar centers.