Effects of Prenatal Exposure to Styrene Trimers on Genital Organs and Hormones in Male Rats
Effects of Prenatal Exposure to Styrene Trimers on Genital Organs and Hormones in Male Rats
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产前接触苯乙烯三聚体对雄性大鼠生殖器官和激素的影响
DOI：
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发表时间：
2007
影响因子：
3.2
通讯作者：
F. Nagai
中科院分区：
文献类型：
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作者：
K. Ohyama;K. Satoh;Y. Sakamoto;A. Ogata;F. Nagai
苯乙烯三聚体从聚苯乙烯食品容器迁移到食品中。我们评价了苯乙烯三聚体如2，4，6-三苯基-1-己烯（ST-1）、1a-苯基-4a-（1'-phenylethyl）tetralin（ST-2），1a-phenyl-4e-（1'-phenylethyl）tetralin（ST-3），1e-phenyl-4a-（1′-苯乙基）四氢化萘（ST-4），和1 e-苯基-4e-（1′-苯乙基）四氢化萘（ST-5），并证实ST-1，ST-3，ST-4具有雌激素样活性。另一方面，ST-2和ST-5具有抗雌激素样活性。我们研究了ST-1、ST-3和ST-4的体内雌激素活性。将苯乙烯三聚体给予妊娠大鼠，并检查其对后代的影响。从妊娠第11天至第17天，对妊娠大鼠皮下注射ST-1、ST-3或ST-4（0、10、100、1000 μg/kg体重/天），并在出生后第101-103天（PND）处死雄性后代。在ST-4治疗组中，PND 3时的相对肛门生殖器距离显著缩短。所有苯乙烯三聚体处理组的相对睾丸重量均显著降低。ST-4给药组中前列腺和附睾的相对重量显著降低。ST-3和ST-4治疗组的相对脑重量显著降低。在ST-1和ST-4处理组中观察到支持细胞计数显著降低。所有苯乙烯三聚体治疗组的血清促卵泡激素水平均显著降低。ST-1和ST-4组的黄体生成素水平显著降低，睾酮水平升高。这些结果表明，产前暴露于雌激素苯乙烯三聚体在低水平阻碍生殖器官的发育，扰乱雄性大鼠后代的内分泌系统。
Styrene trimers migrate from polystyrene food container into foods. We evaluated the estrogenic activity of styrene trimers such as 2,4,6-triphenyl-1-hexene (ST-1), 1a-phenyl-4a-(1′-phenylethyl)tetralin (ST-2), 1a-phenyl-4e-(1′-phenylethyl)tetralin(ST-3), 1e-phenyl-4a-(1′-phenylethyl)tetralin (ST-4), and 1e-phenyl-4e-(1′-phenylethyl)tetralin (ST-5) using the reporter-gene assay with MVLN cells stably expressing the estrogen-stimulated reporter gene, and it was confirmed that ST-1, ST-3, and ST-4 had estrogen-like activity. On the other hand, ST-2 and ST-5 had anti-estrogen-like activity. We examined the estrogenic activity in vivo of ST-1, ST-3, and ST-4. The styrene trimers were administered to pregnant rats, and the effects on the offspring were examined. ST-1, ST-3, or ST-4 (0, 10, 100, 1000 μg/kg body wt/day) were subcutaneously injected into pregnant rats from gestational Day 11 through 17, and the male offspring were sacrificed on postnatal days (PND) 101–103. In the ST-4 treatment groups, the relative anogenital distance on PND 3 was significantly shortened. The relative testis weight was remarkably decreased in all styrene trimer treatment groups. Relative weights of the prostate and epididymides significantly decreased in the ST-4 treatment groups. The relative brain weight was markedly reduced in the ST-3 and ST-4 treatment groups. A significant decrease of the Sertoli cell count was observed in the ST-1 and ST-4 treatment groups. The serum follicle stimulating hormone level was remarkably reduced in all styrene trimer treatment groups. The luteinizing hormone level was significantly decreased and the testosterone level increased in the ST-1 and ST-4 groups. These results suggest that prenatal exposure to estrogenic styrene trimers at low levels obstructed genital organ development, and disrupted the endocrine systems of male rat offspring.
影响因子：
4.8
作者：
ORTH, JM;GUNSALUS, GL;LAMPERTI, AA
通讯作者：
LAMPERTI, AA