Video-supported analysis of Beggiatoa filament growth, breakage, and movement
Video-supported analysis of Beggiatoa filament growth, breakage, and movement
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DOI：
10.1007/s00248-008-9367-x
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发表时间：
2008-10-01
影响因子：
3.6
通讯作者：
Schulz-Vogt, Heide N.
中科院分区：
文献类型：
--
作者：
Kamp, Anja;Roy, Hans;Schulz-Vogt, Heide N.
海洋 Beggiatoa sp.在具有相反的氧-硫化物梯度的半固体琼脂中培养。通过延时视频记录研究了透明琼脂中贝吉亚托亚细丝的生长模式、增殖时细丝的断裂和运动方向。室温下细胞的初始倍增时间为 15.7 +/- 1.3 小时（平均值 +/- SD）。细丝生长到平均长度为 1.7 +/- 0.2 毫米，但也存在长达约 6 毫米的细丝。细丝的第一次断裂发生在接种后大约 19 小时，延时电影显示母体细丝可以在几个小时内断裂成多个子体细丝。在 > 20% 的情况下，细丝断裂发生在前一个环的尖端。由于细丝断裂是通过牺牲细胞的存在来实现的，因此环的形成和牺牲细胞的存在必须一致。我们假设牺牲细胞通过中断一根细丝两部分之间的通讯来增加环形成的机会。当通讯中断时，一根细丝的这两部分可以随机地向彼此移动，在牺牲单元处形成环的尖端。
A marine Beggiatoa sp. was cultured in semi-solid agar with opposing oxygen-sulfide gradients. Growth pattern, breakage of filaments for multiplication, and movement directions of Beggiatoa filaments in the transparent agar were investigated by time-lapse video recording. The initial doubling time of cells was 15.7 +/- 1.3 h (mean +/- SD) at room temperature. Filaments grew up to an average length of 1.7 +/- 0.2 mm, but filaments of up to approximately 6 mm were also present. First breakages of filaments occurred approximately 19 h after inoculation, and time-lapse movies illustrated that a parent filament could break into several daughter filaments within a few hours. In > 20% of the cases, filament breakage occurred at the tip of a former loop. As filament breakage is accomplished by the presence of sacrificial cells, loop formation and the presence of sacrificial cells must coincide. We hypothesize that sacrificial cells enhance the chance of loop formation by interrupting the communication between two parts of one filament. With communication interrupted, these two parts of one filament can randomly move toward each other forming the tip of a loop at the sacrificial cell.