Calorimetric studies of the growth of anaerobic microbes
Calorimetric studies of the growth of anaerobic microbes
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DOI：
10.1016/j.jbiosc.2016.02.006
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发表时间：
2016-09-01
影响因子：
2.8
通讯作者：
Tanaka, Akiyoshi
中科院分区：
文献类型：
--
作者：
Miyake, Hideo;Maeda, Yukiko;Tanaka, Akiyoshi
本文旨在验证量热法测量厌氧微生物生长的使用。一直难以在保持最佳生长条件的同时监测严格厌氧菌的生长。传统上，光密度和ATP浓度通常被用作厌氧微生物生长的量度。然而，为了进行这些测量，有必要提取培养物的等分试样，这在保持厌氧条件的同时可能是困难的。在这项研究中，量热法被用来连续和非破坏性地测量厌氧微生物生长产生的热量作为时间的函数。使用丙酮丁醇梭菌、拜氏梭菌和食纤维梭菌作为代表性厌氧微生物。利用多路等温量热仪，观察到C.随着接种率的降低，丙酮丁醇的放热增加。接种率和t（p）之间的这种强相关性表明，可以通过量热法测量厌氧微生物的生长速率。总的来说，我们的结果表明，放热和光密度/ATP浓度之间有很好的相关性，验证了该方法的使用。(C)2016年，日本生物技术学会。All rights reserved.
This article aims to validate the use of calorimetry to measure the growth of anaerobic microbes. It has been difficult to monitor the growth of strict anaerobes while maintaining optimal growth conditions. Traditionally, optical density and ATP concentration are usually used as measures of the growth of anaerobic microbes. However, to take these measurements it is necessary to extract an aliquot of the culture, which can be difficult while maintaining anaerobic conditions. In this study, calorimetry was used to continuously and nondestructively measure the heat generated by the growth of anaerobic microbes as a function of time. Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium cellulovorans were used as representative anaerobic microbes. Using a multiplex isothermal calorimeter, we observed that peak time (t(p)) of C. acetobutylicum heat evolution increased as the inoculation rate decreased. This strong correlation between the inoculation rate and t(p) showed that it was possible to measure the growth rate of anaerobic microbes by calorimetry. Overall, our results showed that there is a very good correlation between heat evolution and optical density/ATP concentration, validating the use of the method. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.