The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants
The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants
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DOI：
10.1038/sj.embor.7400262
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发表时间：
2004-10-01
期刊：
影响因子：
7.7
通讯作者：
Michel, B
中科院分区：
文献类型：
--
作者：
Flores, MJ;Bidnenko, V;Michel, B
主要的大肠杆菌DNA聚合酶（聚合酶III）的失活所阻止的复制叉通过新合成的前导链和滞后链末端的退火而逆转。反向叉通过RecBC作用于DNA双链末端而重置，并且在不存在RecBC的情况下，染色体通过霍利迪连接解离酶RuvABC线性化。我们在这里报告，UvrD解旋酶是必不可少的RuvABC依赖的染色体线性化在E。大肠杆菌聚合酶III突变体，而它的合作伙伴在DNA修复（UvrA/B和MutL/S）不是。UvrD参与了E.杆菌
Replication forks arrested by inactivation of the main Escherichia coli DNA polymerase (polymerase III) are reversed by the annealing of newly synthesized leading- and lagging-strand ends. Reversed forks are reset by the action of RecBC on the DNA double-strand end, and in the absence of RecBC chromosomes are linearized by the Holliday junction resolvase RuvABC. We report here that the UvrD helicase is essential for RuvABC-dependent chromosome linearization in E. coli polymerase III mutants, whereas its partners in DNA repair (UvrA/B and MutL/S) are not. We conclude that UvrD participates in replication fork reversal in E. coli.