Comparative analysis of the sialic acid binding activity and the tropism for the respiratory epithelium of four different strains of avian infectious bronchitis virus
Comparative analysis of the sialic acid binding activity and the tropism for the respiratory epithelium of four different strains of avian infectious bronchitis virus
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DOI：
10.1080/03079450802632049
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发表时间：
2009-01-01
期刊：
影响因子：
2.8
通讯作者：
Winter, C.
中科院分区：
文献类型：
--
作者：
El Rahman, S. Abd;El-Kenawy, A. A.;Winter, C.
鸡传染性支气管炎病毒（IBV）是危害商品家禽的主要病原。我们最近证明唾液酸作为气管上皮上IBV的受体决定簇。在这里，我们比较了IBV毒株Beaudette、4/91、Italy 02和QX的唾液酸结合特性。我们证明，唾液酸结合是重要的感染原代鸡肾细胞和气管上皮细胞的所有四个菌株。四个毒株之间只有轻微的差异，表明IBV普遍使用唾液酸作为受体决定簇。此外，我们分析了四种不同菌株呼吸道上皮中的主要靶细胞，发现它们都感染了纤毛细胞和杯状细胞。
Avian infectious bronchitis virus (IBV) is a major pathogen in commercial poultry flocks. We recently demonstrated that sialic acid serves as a receptor determinant for IBV on the tracheal epithelium. Here we compared the IBV strains Beaudette, 4/91, Italy02, and QX for their sialic acid-binding properties. We demonstrate that sialic acid binding is important for the infection of primary chicken kidney cells and the tracheal epithelium by all four strains. There were only slight differences between the four strains, indicating the universal usage of sialic acids as receptor determinants by IBV. In addition, we analysed the primary target cells in the respiratory epithelium of the four different strains and found that all of them infected ciliated cells and goblet cells.