Physiochemical Changes to TTCF Ensilication Investigated Using Time-Resolved SAXS
Physiochemical Changes to TTCF Ensilication Investigated Using Time-Resolved SAXS
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DOI：
10.3390/appliedchem1010002
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发表时间：
2021-08
期刊：
影响因子：
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通讯作者：
A. Doekhie;R. Dattani;Yun-Chu Chen;F. Koumanov;K. Edler;J. van den Elsen;A. Sartbaeva
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作者：
A. Doekhie;R. Dattani;Yun-Chu Chen;F. Koumanov;K. Edler;J. van den Elsen;A. Sartbaeva
成功根除或控制流行传染病与疫苗的效力、稳定性和分布有关。大多数基于蛋白质的疫苗在冰箱（2-8 °C）温度下运输，冷链，以保持效力。然而，这已被证明是有问题的。蛋白质天生容易受到运输过程中发生的热波动的影响，导致它们变性。这导致疫苗无效和疫苗可预防疾病的增加，特别是在低收入国家。我们的研究利用二氧化硅在室温下保存疫苗，无需冷链物流。该方法是基于溶胶-凝胶化学，其中可溶性二氧化硅被用来封装和ensilicate疫苗蛋白。这产生了蛋白质负载的二氧化硅纳米颗粒粉末，其在室温下储存，随后使用快速化学过程释放。我们以前已经表明，破伤风毒素C片段（TTCF）的硅化是一个扩散限制簇聚集（DLCA）为基础的过程，使用时间分辨小角X射线散射（SAXS）。在这里，我们提出了我们的扩展调查，这个系统的模块化，以进一步了解硅化通过时间分辨SAXS。我们的研究结果表明，在硅化过程中的变化，可以证明是有用的过渡，从批量到在流制造的硅化纳米粒子。
Successful eradication or control of prevailing infectious diseases is linked to vaccine efficacy, stability, and distribution. The majority of protein-based vaccines are transported at fridge (2–8 °C) temperatures, cold chain, to retain potency. However, this has been shown to be problematic. Proteins are inherently susceptible to thermal fluctuations, occurring during transportation, causing them to denature. This leads to ineffective vaccines and an increase in vaccine-preventable diseases, especially in low-income countries. Our research utilises silica to preserve vaccines at room temperature, removing the need for cold chain logistics. The methodology is based upon sol–gel chemistry in which soluble silica is employed to encapsulate and ensilicate vaccine proteins. This yields a protein-loaded silica nanoparticle powder which is stored at room temperature and subsequently released using a fast chemical process. We have previously shown that tetanus toxin C fragment (TTCF) ensilication is a diffusion-limited cluster aggregation (DLCA)-based process using time-resolved small-angle x-ray scattering (SAXS). Here, we present our expanded investigation on the modularity of this system to further the understanding of ensilication via time-resolved SAXS. Our results show that variations in the ensilication process could prove useful in the transition from batch to in-flow manufacturing of ensilicated nanoparticles.