Antigenic relationships between Bovine viral diarrhea virus 1 and 2 and HoBi virus: Possible impacts on diagnosis and control
Antigenic relationships between Bovine viral diarrhea virus 1 and 2 and HoBi virus: Possible impacts on diagnosis and control
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DOI：
10.1177/1040638711435144
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发表时间：
2012-03-01
影响因子：
1.5
通讯作者：
Ridpath, Julia F.
中科院分区：
文献类型：
--
作者：
Bauermann, Fernando V.;Flores, Eduardo F.;Ridpath, Julia F.
在牛中出现新发现的一类鼠疫病毒，即霍比病毒样病毒，需要对现有诊断工具和疫苗进行评估。本研究比较了牛病毒性腹泻病毒1、2株（BVDV-1、-2）与霍比病毒的抗原特性。这种比较是基于商业酶联免疫吸附试验（elisa）检测HoBi病毒及其抗体，以及接种BVDV的动物血清中存在的交叉中和抗体水平。与一组单克隆抗体（mab）的反应性显示，BVDV物种（BVDV-1, -2）与E-rns和NS2/3蛋白内的HoBi表位之间的交叉反应性比E2糖蛋白表位之间的交叉反应性更强。结果表明，基于E-rns或NS2/3表位设计的BVDV和HoBi的诊断试验可用于鼠疫病毒种间分化试验，而E2表位的差异可用于鼠疫病毒种间分化试验。基于E-rns检测的抗原捕获ELISA试剂盒检测HoBi病毒的阈值与BVDV的阈值具有统计学意义相似。相比之下，用于检测BVDV抗体的2种商用ELISA试剂盒对含有HoBi病毒中和抗体的血清样品的检测分别漏检22.2%和77.7%。此外，接种BVDV-1和-2的犊牛血清对霍比病毒的中和活性较低。结果表明，尽管在抗原性上存在相似性，但HoBi病毒在抗原性上与这两种BVDV不同。因此，检测和控制牛的霍比病毒感染将需要开发新的诊断试剂和重新配制现有疫苗。
The emergence of a newly recognized group of pestiviruses in cattle, the HoBi-like viruses, requires an evaluation of the available diagnostic tools and vaccines. The present study compared antigenic characteristics of Bovine viral diarrhea virus 1 and 2 (BVDV-1, -2) strains and HoBi virus. This comparison was based on detection of HoBi virus and antibodies against it by commercial enzyme-linked immunosorbent assays (ELISAs) and the level of cross-neutralizing antibodies present in sera from animals vaccinated with BVDV. Reactivity with a panel of monoclonal antibodies (mAbs) revealed greater cross-reactivity between BVDV species (BVDV-1, -2) and HoBi epitopes within E-rns and NS2/3 proteins than between epitopes located in the E2 glycoprotein. The results suggest that a diagnostic test designed to detect both BVDV species and HoBi could be based on E-rns or NS2/3 epitopes, while variation among E2 epitopes could be exploited in tests for differentiation of pestivirus species. The threshold of detection of HoBi virus by an antigen-capture ELISA kit based on detection of E-rns was statistically similar to that for BVDV. In contrast, 2 commercial ELISA kits designed to detect antibodies against BVDV missed 22.2% and 77.7%, respectively, of serum samples harboring HoBi virus-neutralizing antibodies. In addition, sera of calves vaccinated with BVDV-1 and -2 presented low neutralizing activity against HoBi virus. The results demonstrate that in spite of antigenic similarities, HoBi virus is antigenically distinct from both BVDV species. Detection and control of HoBi virus infections in cattle would thus require the development of new diagnostic reagents and reformulation of current vaccines.