Mutations that suppress the deletion of an upstream activating sequence in yeast: involvement of a protein kinase and histone H3 in repressing transcription in vivo.
Mutations that suppress the deletion of an upstream activating sequence in yeast: involvement of a protein kinase and histone H3 in repressing transcription in vivo.
复制标题
抑制酵母上游激活序列缺失的突变：蛋白激酶和组蛋白 H3 参与抑制体内转录。
DOI：
10.1093/genetics/135.3.665
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发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
Winston,F
中科院分区：
文献类型：
--
作者：
Prelich,G;Winston,F
酿酒酵母中大多数蛋白质编码基因的转录调控需要上游激活序列（UAS）;在缺乏UAS元件的情况下，很少或根本没有转录发生。然而，在某些突变株中，已经删除其UAS的启动子可以指导显著水平的转录，这表明剩余的启动子元件（基础启动子）能够指导更高水平的转录，但它们通常在野生型菌株中存在。为了分析这种抑制，我们选择了在不存在UAS的情况下导致SUC 2基因转录增加的突变。除了一些以前研究的基因，这个选择已经确定了五个基因，我们已经指定BUR 1，BUR 2，BUR 3，BUR 5和BUR 6（旁路UAS要求）。bur突变引起多效性表型，表明它们影响许多基因的转录。此外，一些bur突变抑制在SUC 2和Ty处对SNF 5反式激活因子的需求。进一步的分析表明，BUR 1与SGV 1相同，后者编码一种与CDC 28相关的蛋白激酶。这一结果表明，蛋白磷酸化对于SUC 2基础启动子的抑制以及体内转录的其他方面是重要的。最后，BUR 5与HHT 1相同，编码组蛋白H3，进一步暗示染色质结构对于SUC 2的表达是重要的。
Regulated transcription of most protein-encoding genes in Saccharomyces cerevisiae requires an upstream activating sequence (UAS); in the absence of UAS elements, little or no transcription occurs. In certain mutant strains, however, promoters that have been deleted for their UAS can direct significant levels of transcription, indicating that the remaining promoter elements (the basal promoter) are capable of directing higher levels of transcription, but they are normally represented in wild-type strains. To analyze this repression, we have selected for mutations that cause increased transcription of the SUC2 gene in the absence of its UAS. In addition to some previously studied genes, this selection has identified five genes that we have designated BUR1, BUR2, BUR3, BUR5 and BUR6 (for Bypass UAS Requirement). The bur mutations cause pleiotropic phenotypes, indicating that they affect transcription of many genes. Furthermore, some bur mutations suppress the requirement for the SNF5 trans-activator at both SUC2 and Ty. Additional analysis has demonstrated that BUR1 is identical to SGV1, which encodes a CDC28-related protein kinase. This result indicates that protein phosphorylation is important for repression of the SUC2 basal promoter as well as other aspects of transcription in vivo. Finally, BUR5 is identical to HHT1, encoding histone H3, further implicating chromatin structure as important for expression of SUC2.