Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas
Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas
复制标题
Illumina MiSeq测序对比不同铁矿区土壤细菌群落结构研究
DOI：
10.1007/s11356-015-4186-3
复制
发表时间：
2015-07-01
影响因子：
5.8
通讯作者：
Li, Yang
中科院分区：
文献类型：
--
作者：
Hong, Chen;Si, Yanxiao;Li, Yang
采矿活动将重金属泄漏到周围的土壤中，可能会影响土著微生物群落。采用Illumina MiSeq测序技术，对北京市潮河流域重污染、中度污染和非污染3个不同污染程度区域土壤细菌群落的多样性和组成进行了比较。稀疏化结果表明，污染区的细菌α多样性显著高于未污染区。主成分分析（PCA）结果表明，污染区微生物群落与未污染区相比存在显著差异。此外，在门水平上的PCA和Matastats的结果表明，在不同地点的群落具有相似的门的多样性，表明金属污染下的细菌群落的变化并没有反映在门结构。在属的水平上，优势属的相对多度随污染程度的不同而变化。慢生根瘤菌属（Bradyrhizobium）、Rhodanophyll、Reyranella和Rhizomicrobium等4个属的优势度随着污染程度的增加而显著下降，在重金属污染区，Steroidobacter、Massilia、Arthrobacter、Flavisolibacter和Roseiflexus等优势菌属数量增加，成为新的优势菌属。在土壤生态系统中起重要作用的土著细菌群落中，潜在的耐药细菌主要分布在硫杆菌属（Thiobacillus）、假单胞菌属（Pseudomonas）、节杆菌属（Arthrobacter）、微鞘菌属（Microcoleus）、细喉囊菌属（Leptolyngbya）和红杆菌属（Rhodobacter）中，其比例不到2.0%。这一背景多样性剖面的研究为深入了解轻度重金属污染下群落结构变化的机制奠定了基础。
Mine activities leaked heavy metals into surrounding soil and may affected indigenous microbial communities. In the present study, the diversity and composition of the bacterial community in soil collected from three regions which have different pollution degree, heavy pollution, moderate pollution, and non-pollution, within the catchment of Chao River in Beijing City, were compared using the Illumina MiSeq sequencing technique. Rarefaction results showed that the polluted area had significant higher bacterial alpha diversity than those from unpolluted area. Principal component analysis (PCA) showed that microbial communities in the polluted areas had significant differences compared with the unpolluted area. Moreover, PCA at phylum level and Matastats results demonstrated that communities in locations shared similar phyla diversity, indicating that the bacterial community changes under metal pollution were not reflected on phyla structure. At genus level, the relative abundance of dominant genera changed in sites with degrees of pollution. GeneraBradyrhizobium,Rhodanobacter,Reyranella, andRhizomicrobiumsignificantly decreased with increasing pollution degree, and their dominance decreased, whereas several genera (e.g.,Steroidobacter,Massilia,Arthrobacter,Flavisolibacter, andRoseiflexus) increased and became new dominant genera in the heavily metal-polluted area. The potential resistant bacteria, found within the genera ofThiobacillus,Pseudomonas,Arthrobacter,Microcoleus,Leptolyngbya, andRhodobacter, are less than 2.0 % in the indigenous bacterial communities, which play an important role in soil ecosystem. This effort to profile the background diversity may set the first stage for better understanding the mechanism underlying the community structure changes under in situ mild heavy metal pollution.