Near-Single-Cell Proteomics Profiling of the Proximal Tubular and Glomerulus of the Normal Human Kidney.
Near-Single-Cell Proteomics Profiling of the Proximal Tubular and Glomerulus of the Normal Human Kidney.
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DOI：
10.3389/fmed.2020.00499
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发表时间：
2020
影响因子：
3.9
通讯作者：
Kidney Precision Medicine Project (KPMP) Consortium
中科院分区：
文献类型：
--
作者：
Sigdel TK;Piehowski PD;Roy S;Liberto J;Hansen JR;Swensen AC;Zhao R;Zhu Y;Rashmi P;Schroeder A;Damm I;Sur S;Luo J;Yang Y;Qian WJ;Sarwal MM;Kidney Precision Medicine Project (KPMP) Consortium
单细胞水平的分子评估可以通过提供疾病和健康中细胞组织和组织异质性的详细评估来加速生物学研究。人类肾脏具有复杂的多细胞状态和不同的功能，其中大部分现在可以通过近单细胞水平的组织蛋白质组学的最新技术进步来完全利用。作为肾脏精准医学项目 (KPMP) 的一部分，我们讨论了首次应用这种基于质谱 (MS) 的蛋白质组学方法来分析正常人肾脏的子部分的基本步骤。使用约 30-40 个激光捕获的显微解剖肾细胞，我们鉴定了超过 2,500 种人类蛋白质，这些蛋白质对肾脏的近端肾小管（PT；n = 25 种蛋白质）和肾小球（Glom；n = 67 种蛋白质）区域具有特异性及其独特的代谢功能。这项试点研究为应用我们的近单细胞蛋白质组学工作流程在更大范围内分析其他肾脏微区室提供了路线图，以揭示正常肾脏中肾亚细胞功能的扰动以及急性和慢性肾脏疾病的不同病因。
Molecular assessments at the single cell level can accelerate biological research by providing detailed assessments of cellular organization and tissue heterogeneity in both disease and health. The human kidney has complex multi-cellular states with varying functionality, much of which can now be completely harnessed with recent technological advances in tissue proteomics at a near single-cell level. We discuss the foundational steps in the first application of this mass spectrometry (MS) based proteomics method for analysis of sub-sections of the normal human kidney, as part of the Kidney Precision Medicine Project (KPMP). Using ~30–40 laser captured micro-dissected kidney cells, we identified more than 2,500 human proteins, with specificity to the proximal tubular (PT; n = 25 proteins) and glomerular (Glom; n = 67 proteins) regions of the kidney and their unique metabolic functions. This pilot study provides the roadmap for application of our near-single-cell proteomics workflow for analysis of other renal micro-compartments, on a larger scale, to unravel perturbations of renal sub-cellular function in the normal kidney as well as different etiologies of acute and chronic kidney disease.
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影响因子：
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通讯作者：
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