The Increased RNase Activity of I RE1α in PBMCs from Patients with Rheumatoid Arthritis
The Increased RNase Activity of I RE1α in PBMCs from Patients with Rheumatoid Arthritis
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DOI：
10.15171/apb.2019.060
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发表时间：
2019-01-01
影响因子：
3.6
通讯作者：
Rahmati, Marveh
中科院分区：
文献类型：
--
作者：
Ahmadiany, Mahdieh;Alavi-Samani, Mahshid;Rahmati, Marveh
Purpose: Despite recent advances in the diagnosis and treatment of rheumatoid arthritis (RA), this inflammatory disease remains a challenge to patients and physicians. Recent evidence highlights the contribution of endoplasmic reticulum (ER) stress in the pathogenesis and treatment of RA. Herein, we study the expression of the ER stress sensor inositol-requiring enzyme 1 alpha (IRE1 alpha), as well as XBP1 splicing and the regulated IRE1-dependent decay (RIDD), in peripheral blood mononuclear cells (PBMCs) from patients with RA compared with healthy controls.Methods: The PBMCs from blood samples of RA patients and healthy volunteers were isolated by a density gradient centrifugation method using Ficoll. The gene expression levels of GRP78/Bip, IRE1, XBP1s, micro-RNAs (miRNAs) were evaluated by real-time PCR.Results: The expression of GRP78, IRE1, and XBP1s were increased in PBMCs of RA patients compared with healthy controls. We further show that the RIDD targets (miRNA-17, -34a, -96, and -125b) were downregulated in RA samples.Conclusion: This study can expand our knowledge on the importance of RNase activity of IRE1 alpha in RA and may offer new potentials for developing novel diagnostic and/or therapeutic biomarkers.
