Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection
Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection
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DOI：
10.1073/pnas.0502950102
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发表时间：
2005-09-13
影响因子：
11.1
通讯作者：
Ohta, T
中科院分区：
文献类型：
--
作者：
Kuroda, M;Yamashita, A;Ohta, T
腐生葡萄球菌是一种泌尿道致病性葡萄球菌，经常从患有单纯性尿路感染的年轻女性门诊患者中分离出来。我们对腐生沙门氏菌型菌株 ATCC 15305 的全基因组进行了测序，该菌株含有 2,516,575 bp 的环状染色体、2,446 个 ORF 和两个质粒。与另外两个物种金黄色葡萄球菌和表皮葡萄球菌的菌株的比较基因组分析以及实验数据揭示了腐生葡萄球菌基因组的以下特征。腐生链球菌不具有金黄色葡萄球菌中发现的任何毒力因子，例如凝固酶、肠毒素、胞外酶和细胞外基质结合蛋白，尽管它确实具有与泌尿环境中高度可变的离子含量相关的运输系统的显着旁系同源扩张。另一个独特的特征是，只有一个 ORF 可预测为细胞壁锚定蛋白，并且它显示出阳性血凝和与尿道中初始定植相关的人膀胱细胞的粘附。它还在腐生链球菌中显示出显着高的脲酶活性。腐生沙门氏菌的泌尿道致病性可归因于其基因组，该基因组是其通过新型细胞壁锚定粘附素和多余的泌尿适应性转运系统以及脲酶在人类泌尿道中生存所必需的。
Staphylococcus saprophyticus is a uropathogenic Staphylococcus frequently isolated from young female outpatients presenting with uncomplicated urinary tract infections. We sequenced the whole genome of S. saprophyticus type strain ATCC 15305, which harbors a circular chromosome of 2,516,575 bp with 2,446 ORFs and two plasmids. Comparative genomic analyses with the strains of two other species, Staphylococcus aureus and Staphylococcus epidermidis, as well as experimental data, revealed the following characteristics of the S. saprophyticus genome. S. saprophyticus does not possess any virulence factors found in S. aureus, such as coagulase, enterotoxins, exoenzymes, and extracellular matrix-binding proteins, although it does have a remarkable paralog expansion of transport systems related to highly variable ion contents in the urinary environment. A further unique feature is that only a single ORF is predictable as a cell wall-anchored protein, and it shows positive hemagglutination and adherence to human bladder cell associated with initial colonization in the urinary tract. It also shows significantly high urease activity in S. saprophyticus. The uropathogenicity of S. saprophyticus can be attributed to its genome that is needed for its survival in the human urinary tract by means of novel cell wall-anchored adhesin and redundant uro-adaptive transport systems, together with urease.