A graph theory method for determination of cryo-EM image focuses.
A graph theory method for determination of cryo-EM image focuses.
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DOI：
10.1016/j.jsb.2012.07.005
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发表时间：
2012-11
影响因子：
3
通讯作者：
Liu, Zheng
中科院分区：
文献类型：
--
作者：
Jiang, Wen;Guo, Fei;Liu, Zheng
精确确定显微图像的焦点是至关重要的平均多个图像，以达到高分辨率的3-D重建在电子冷冻显微镜（cryo-EM）。目前的方法使用迭代拟合的焦点相关的模拟功率谱的实验图像的功率谱，与拟合独立地执行不同的图像。在这里，我们已经开发了一种新的图论为基础的方法，其中所有图像的旋转平均焦点和个人的角度扇区焦点同时确定在封闭的形式使用超定线性方程组的最小二乘解。新方法被证明是快速，准确，和强大的测试与实验低剂量冷冻EM图像的大数据集。它与三种经典的功率谱拟合方法的集成也允许通过这些截然不同的方法对结果进行交叉验证。新的集成焦点确定方法将提高大规模数据处理的自动焦点确定的可靠性，这在低温EM领域越来越普遍。
Accurate determination of micrograph focuses is essential for averaging multiple images to reach high-resolution 3-D reconstructions in electron cryo-microscopy (cryo-EM). Current methods use iterative fitting of focus-dependent simulated power spectra to the power spectra of experimental images, with the fitting performed independently for different images. Here we have developed a novel graph theory based method in which the rotational average focus and individual angular sector focuses of all images are determined simultaneously in closed form using the least square solution of overdetermined linear equations. The new method was shown to be fast, accurate, and robust in tests with large datasets of experimental low dose cryo-EM images. Its integration with three classic power spectra fitting methods also allows cross validation of the results by these vastly different methods. The new integrated focus determination method will improve reliability of automated focus determination for large-scale data processing that is increasingly common in the cryo-EM field.
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