RNA-Seq Reveals miRNA Role Shifts in Seven Stages of Skeletal Muscles in Goat Fetuses and Kids
RNA-Seq Reveals miRNA Role Shifts in Seven Stages of Skeletal Muscles in Goat Fetuses and Kids
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RNA-Seq 揭示了山羊胎儿和儿童骨骼肌七个阶段中 miRNA 的作用转变
DOI：
10.3389/fgene.2020.00684
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发表时间：
2020-07-07
影响因子：
3.7
通讯作者：
Zhang, Xiaorong
中科院分区：
文献类型：
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作者：
Ling, Yinghui;Zheng, Qi;Zhang, Xiaorong
MicroRNAs （miRNAs）在骨骼肌的调控中是不可或缺的。我们进行了RNA测序（RNA-seq），以建立山羊7个阶段的全面miRNA谱，即45- (F45)、65- (F65)、90- (F90)、120- (F120)和135天（F135）的胎儿、新生儿（B1）和90日龄（B90）的婴儿。数据中共鉴定出421种已知mirna和228种山羊新mirna，其中19种mirna在7个阶段的平均丰度超过10,000 reads / million。此外，在所有对照组中，在7个阶段鉴定出420个差异表达的mirna (demirna)，其中80个在B1和B90对照组中唯一差异表达。通路分析表明，该组与肌肉肥大的释放和成肌细胞增殖的调节有关。此外，305个demirna被聚集成3个显著富集的谱（谱11、16和19）。功能分析显示，基因型16与肌肉肥大和分化有关。Profile 11参与了肌肉细胞的多种酶活性和代谢过程。剖面19与物质传输和结构稳定性有关。通过实时荧光定量PCR验证这些基因图谱中的2个高表达mirna和3个关键mirna （chi-miR-328-3p、chi-miR-767和chi-miR-150）与数据一致。这些结果提供了山羊肌肉相关miRNA的目录，使我们能够更好地了解哺乳动物肌肉发育过程中miRNA作用的转化。
MicroRNAs (miRNAs) are indispensable for the regulation of skeletal muscle. We performed RNA sequencing (RNA-seq) to establish a comprehensive miRNA profiling of goats in seven stages, namely, 45- (F45), 65- (F65), 90- (F90), 120- (F120), and 135-day (F135) fetuses, newborn (B1), and 90-day-old (B90) kids. In total, 421 known miRNAs and 228 goat novel miRNAs were identified in the data, and the average abundance of 19 miRNAs in seven stages exceeds 10,000 reads per million. Furthermore, 420 differentially expressed miRNAs (DEmiRNAs) were identified in all comparison group at seven stages, 80 of which were uniquely differentially expressed in the B1 and B90 comparison groups. Pathway analysis indicated that this group was associated with the release of muscle hypertrophy and regulation of myoblast proliferation. Besides, 305 DEmiRNAs were clustered into three significantly enriched profiles (profiles 11, 16, and 19). Function analysis revealed that profile 16 was related to muscle hypertrophy and differentiation. Profile 11 was involved in multiple enzyme activities and metabolic processes in muscle cells. And profile 19 was involved in material transport and structural stability. Two highly expressed miRNAs and three key miRNAs (chi-miR-328-3p, chi-miR-767, and chi-miR-150) of these profiles were verified to be consistent with the data by quantitative real-time PCR. These results provided a catalog of goat muscle-associated miRNAs, allowing us to better understand the transformation of miRNA roles during mammalian muscle development.