SWI/SNF has intrinsic nucleosome disassembly activity that is dependent on adjacent nucleosomes.
SWI/SNF has intrinsic nucleosome disassembly activity that is dependent on adjacent nucleosomes.
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DOI：
10.1016/j.molcel.2010.02.040
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发表时间：
2010-05-28
期刊：
影响因子：
16
通讯作者：
Bartholomew B
中科院分区：
文献类型：
--
作者：
Dechassa ML;Sabri A;Pondugula S;Kassabov SR;Chatterjee N;Kladde MP;Bartholomew B
ATP依赖的染色质重塑复合物SWI/SNF调节转录，并与启动子核小体驱逐有关。然而，在生物化学测定中单独通过SWI/SNF的有效核小体分解尚未直接观察到。采用一个模型系统的dinucleosomes，而不是单核细胞，我们表明，重塑导致有序和有效的拆卸的两个核小体之一。H2A/H2B二聚体首先被快速置换，然后在较慢的反应中整个组蛋白八聚体丢失。核小体解体SWI/SNF不需要额外的因素，如伴侣或受体的组蛋白。对单个分子的观察以及大量的测量表明，在这个过程中的一个关键中间体是一个核小体向相邻的核小体移动。SWI/SNF由转录激活因子Gal 4-VP 16募集，优先动员近端核小体并使相邻核小体不稳定。
The ATP-dependent chromatin remodeling complex SWI/SNF regulates transcription and has been implicated in promoter nucleosome eviction. Efficient nucleosome disassembly by SWI/SNF alone in biochemical assays has however not been directly observed. Employing a model system of dinucleosomes rather than mononucleosomes, we demonstrate that remodeling leads to ordered and efficient disassembly of one of the two nucleosomes. An H2A/H2B dimer is first rapidly displaced and then in a slower reaction an entire histone octamer is lost. Nucleosome disassembly by SWI/SNF did not require additional factors such as chaperones or acceptors of histones. Observations in single molecules as well as bulk measurement suggest that a key intermediate in this process is one in which a nucleosome is moved towards the adjacent nucleosome. SWI/SNF recruited by the transcriptional activator Gal4-VP16 preferentially mobilizes the proximal nucleosome and destabilizes the adjacent nucleosome.
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影响因子：
16.8
作者：
通讯作者：
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影响因子：
11.4
作者：
Kagalwala, MN;Glaus, BJ;Bartholomew, B
通讯作者：
Bartholomew, B
影响因子：
4.8
作者：
Korber, P;Barbaric, S;Hörz, W
通讯作者：
Hörz, W
哺乳动物启动子的足迹：使用CpG DNA甲基转移酶在单个分子水平上揭示了核小体位置。
影响因子：
14.9
作者：
Fatemi, M;Pao, MM;Jeong, S;Gal-Yam, EN;Egger, G;Weisenberger, DJ;Jones, PA
通讯作者：
Jones, PA
DOI：
10.1073/pnas.95.9.4947
发表时间：
1998-04-28
影响因子：
11.1
作者：
Côté, J;Peterson, CL;Workman, JL
通讯作者：
Workman, JL